Product Specifications for Recombinant Mouse Syndecan-3 Protein, CF
Mouse myeloma cell line, NS0-derived mouse Syndecan-3 protein Ala45-Glu384, with a C-terminal 6-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
90 ‑ 190 kDa, reducing conditions
Measured by the ability of the immobilized protein to inhibit the adhesion of A431 human epithelial carcinoma cells. When 5 x 104 cells/well are added to mouse Syndecan-3 and human Fibronectin (0.5 μg/mL, Catalog # 1918-FN) coated plates, cell adhesion is inhibited in a dose dependent manner after 90 minutes at 37 °C. The ED50 for this effect is 0.2-0.6 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Syndecan-3, also called N-syndecan, is one of four vertebrate syndecans that are principal carriers of heparan sulfate and chondroitin sulfate glycosaminoglycans (GAGs) (1 - 3). These type 1 transmembrane proteins show conserved cytoplasmic domains and divergent extracellular domains (1 - 3). Human Syndecan-3 is synthesized as a 442 amino acid (aa) core protein with a 44 aa signal sequence, a 343 aa extracellular domain (ECD), a 21 aa transmembrane (TM) region and a 34 aa cytoplasmic tail with a binding site for PDZ domains (1). The ECD of mouse Syndecan-3 shares 95%, 83%, 82% and 81% aa identity with of rat, human, canine and bovine Syndecan-3, respectively. Syndecan-3 contains four conserved closely-spaced GAG attachment sites near the N‑terminus and unique threonine-rich and mucin-like sequences near the membrane (4). Addition of glycan side chains results in an apparent size of 120 ‑ 150 kDa. Non-covalent homodimerization of Syndecan-3 or, potentially, heterodimerization with Syndecan-2 or -4, is dependent on the transmembrane domain (5). A cleavage site near the TM domain allows shedding of soluble ECD; the remainder of the molecule undergoes regulated intramembrane proteolysis (6). Syndecan-3 is expressed in the nervous system and at limb buds during development (1, 2). It is expressed on neuronal axons and Schwann cell perinodal processes, promoting nerve cell migration and synapse formation (7, 8). Roles in memory and body weight regulation have been described (2, 9, 10). Through localization of growth factors such as FGF2, HGF and TGF-beta, it regulates expression of molecules important for differentiation of muscle and bone, such as myogenin, BMP-2 and hedgehog family members (1, 2, 11 ‑ 13). In adults, it is up‑regulated during regeneration, such as following myocardial infarction (14).
Xian, X. et al. (2010) Cell Tissue Res. 339:31.
Reizes, O. et al. (2008) Int. J. Biochem. Cell Biol. 40:28.
Carey, D.J. et al. (1997) J. Biol. Chem. 272:2873.
ENTREZ protein Accession # Q64519.
Dews, I.C. and K.R. MacKenzie (2007) Proc. Natl. Acad. Sci. USA 104:20782.
Schultz, J.G. et al. (2003) J. Biol. Chem. 278:48651.
Hienola, A. et al. (2006) J. Cell Biol. 174:569.
Goutebroze, L. et al. (2003) BMC Neurosci. 4:29.
Kaksonen, M. et al. (2002) Mol. Cell. Neurosci. 21:158.
Strader. A.D. et al. (2004) J. Clin. Invest. 114:1354.
Cornelison, D.D.W. et al. (2004) Genes Dev. 18:2231.
Fisher, M.C. et al. (2006) Matrix Biol. 25:27.
Pacifici, M. et al. (2005) J. Bone Miner. Metab. 23:191.
Finsen, A.V. et al. (2004) Physiol. Genomics 16:301.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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