Recombinant Mouse Sulfamidase/SGSH Protein, CF
R&D Systems, part of Bio-Techne | Catalog # 2969-SU
Key Product Details
- R&D Systems Sf 21 (baculovirus)-derived Recombinant Mouse Sulfamidase/SGSH Protein (2969-SU)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Sf 21 (baculovirus)
Accession #
Conjugate
Unconjugated
Applications
Enzyme Activity
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Sulfamidase/SGSH protein
Arg23-Leu502, with a C-terminal 10-His tag
Arg23-Leu502, with a C-terminal 10-His tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal Sequence Analysis
Arg23
Predicted Molecular Mass
56 kDa
SDS-PAGE
60 kDa, reducing conditions
Activity
Measured by its ability to hydrolyze the substrate 4-Nitrocatechol Sulfate (PNCS).
The specific activity is >0.25 pmol/min/µg, as measured under the described conditions.
The specific activity is >0.25 pmol/min/µg, as measured under the described conditions.
Formulation, Preparation and Storage
2969-SU
| Formulation | Supplied as a 0.2 μm filtered solution in Tris and NaCl. |
| Shipping | The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: Sulfamidase/SGSH
Also known as N-sulfoglucosamine sulfohydrolase and heparan N-sulfatase, Sulfamidase/SGSH is an important member of the sulfatase family involved in the degradation of heparan sulfate (1). The SGSH deficiency results in mucopolysaccharidosis type IIIA (MPS IIIA, Sanfilippo A syndrome), an autosomal recessive lysosomal storage disease characterized by neurological dysfunction but relatively mild somatic manifestations (2). The deduced amino acid sequence of mouse SGSH consists of a signal peptide (residues 1-22) and a mature chain (residues 23-502) (1). Recombinant Mouse Sulfamidase/SGSH corresponds to the mature chain and has sulfatase activity as described in the Activity section.
References
- Diez-Roux, G. and Ballabio, A. (2005) Annu. Rev. Genomics Hum. Genet. 6:355.
- Gliddon, B.L. et al. (2004) Mol. Genet. Metab. 83:239.
Alternate Names
HSS, MPS3A, SFMD, SGSH
Gene Symbol
SGSH
UniProt
Additional Sulfamidase/SGSH Products
Product Documents for Recombinant Mouse Sulfamidase/SGSH Protein, CF
Product Specific Notices for Recombinant Mouse Sulfamidase/SGSH Protein, CF
For research use only
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