Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

Name: Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 1004-MR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1004-MR

Citations (9)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P14719
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity

View Full Technical Details for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse ST2/IL-33R protein

Mouse ST2 (Ser27-Ser342) Accession # P14719	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser27

Predicted Molecular Mass

63 kDa (monomer)

SDS-PAGE

73 kDa, reducing conditions

Activity

Measured by its ability to bind Recombinant Mouse IL‑33 (Catalog # 3626-ML) in a functional ELISA with an estimated K_D < 50 nM. Schmitz, J. et al. (2005) Immunity 23:479.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1004-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: ST2/IL-33R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4-7). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11-13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2 (9, 12-14).

Item	Value
References	Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83. Schmitz, J. et al. (2005) Immunity 23:479. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979. Brint, E.K. et al. (2004) Nat. Immunol. 5:373. Weinberg, E.O. et al. (2002) Circulation 106:2961. Sanada S. et al. (2007) J. Clin. Invest. 117:1538 Palmer, G. et al. (2008) Cytokine 42:358. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723. Miller, A.M. et al. (2008) J. Exp. Med. 205:339. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name	Interleukin 33 Receptor
Entrez Gene IDs	9173 (Human); 17082 (Mouse)
Alternate Names	DER4, DER4ST2growth stimulation-expressed, Fit-1, IL-1 R4, IL-1R4, IL-1RL1, IL-33 R, IL-33R, IL1R4, IL33R, Ly84, MGC32623, Protein ST2, ST2, ST2L, ST2V, T1, T1homolog of mouse growth stimulation-expressed, interleukin 1 receptor-like 1, interleukin 1 receptor-related protein, interleukin-1 receptor-like 1

Citations for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF (9)

Showing 1 - 9 of 9 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: Bioassay

LD Faustino et al. (2020), Interleukin-33 activates regulatory T cells to suppress innate &gamma&delta T cell responses in the lung Nat Immunol, 0(0).
PMID: 32989331
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

AL Frisbee et al. (2019), IL-33 drives group 2 innate lymphoid cell-mediated protection during Clostridium difficile infection Nat Commun, 10(1):2712.
PMID: 31221971
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

AK Fu et al. (2016), IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline Proc Natl Acad Sci USA, 0(0).
PMID: 27091974
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Liu T et al. (2016), Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway. J Immunol, 195(8):3537-45.
PMID: 26342029
Species: Rabbit
Sample Types: Cell Culture Supernates
Applications: Bioassay

Seeber S et al. (2014), A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood. PLoS ONE, 9(2):e86184.
PMID: 24503933
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Williams et al. (2013), Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation. Nat Commun, 4(0):2990.
PMID: 24356538
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Drube S et al. (2010), The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells. Blood, 115(19):3899-906.
PMID: 20200353
Species: Mouse
Sample Types: Recombinant Protein
Applications: ELISA (Capture)

Chackerian AA et al. (2007), IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex. J. Immunol., 179(4):2551-5.
PMID: 17675517
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ali S et al. (2007), IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells. Proc. Natl. Acad. Sci. U.S.A., 104(47):18660-5.
PMID: 18003919

There are no citations that match your criteria.

Showing 1 - 9 of 9 citations Showing All

Product Documents for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased