Recombinant Mouse SOST/Sclerostin Protein

Name: Recombinant Mouse SOST/Sclerostin Protein
Brand: R&D Systems
SKU: 1589-ST
Price: 394 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 1589-ST

Citations (6)

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Key Product Details

Source:	NS0
Accession #:	NP_077769
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse SOST/Sclerostin Protein

Product Specifications for Recombinant Mouse SOST/Sclerostin Protein

Source	Mouse myeloma cell line, NS0-derived mouse SOST/Sclerostin protein Gln24-Tyr211 with an N-terminal 7-His tag
Purity	>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	His
Predicted Molecular Mass	22 kDa
SDS-PAGE	30 kDa, reducing conditions
Activity	Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀for this effect is 1-5 μg/mL in the presence of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1589-ST

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1589-ST/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: SOST/Sclerostin

SOST, also known as sclerostin, is a member of the cerberus/DAN family, a group of secreted glycoproteins characterized by a cysteine-knot motif. Cerberus/DAN family members are putative BMP antagonists, and include Dan, Cerberus, Gremlin, PRDC, and Caronte. While the overall sequence identity between members of the family is low, they have conserved spacing of six cysteine residues. Cerberus and Dan have an additional cysteine residue used for dimerization; however, SOST does not and is secreted as a monomer. SOST was originally identified as an important regulator of bone homeostasis. Positional cloning studies identified that mutations in the SOST gene can cause sclerosteosis and van Buchem disease, bone dysplasia disorders characterized by progressive skeletal overgrowth. Significant levels of SOST expression are detected in bone, cartilage, kidney, and liver. SOST is expressed by osteoclasts in developing bones of mouse embryos, including both intramembranously forming skull bones and endochondrally forming long bones. SOST plays a physiological role as a negative regulator of bone formation by repressing BMP-induced osteogenesis. SOST has been shown to have unique ligand specificity, binding BMP-5, -6, and -7 with high affinity and BMP-2 and -4 with low affinity. This seems to be the first example of a BMP antagonist being localized to osteoclasts, cells derived from the hematopoietic lineage, that function to degrade bone matrix. Recombinant human SOST preparations from R&D Systems bind BMP-5 and BMP-6 in a functional ELISA. Human and mouse SOST share 88% amino acid identity (1-3).

Item	Value
References	Kusu, N. et al. (2003) J. Biol. Chem. 278:24113. Balemans, W. et al. (2001) Hum. Mol. Genet. 10:537. Brunkow, M.E. et al. (2001) Am. J. Hum. Genet. 68:577.
Entrez Gene IDs	50964 (Human); 74499 (Mouse)
Alternate Names	SOST, VBCHsclerosteosis, sclerostin

Citations for Recombinant Mouse SOST/Sclerostin Protein (6)

Showing 1 - 6 of 6 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse SOST/Sclerostin Protein include:

Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

K Miyatake et al. (2020), Sclerostin inhibits interleukin-1&beta-induced late stage chondrogenic differentiation through downregulation of Wnt/&beta-catenin signaling pathway PLoS ONE, 15(9):e0239651.
PMID: 32976505
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay

RC Nordberg et al. (2019), LRP receptors in chondrocytes are modulated by simulated microgravity and cyclic hydrostatic pressure PLoS ONE, 14(10):e0223245.
PMID: 31584963
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Y Yamaguchi et al. (2018), Sclerostin is upregulated in the early stage of chondrogenic differentiation, but not required in endochondral ossification in vitro PLoS ONE, 13(8):e0201839.
PMID: 30071108
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

MG Johnson et al. (2017), Endothelin signaling regulates mineralization and posttranscriptionally regulates SOST in TMOb cells via miR 126-3p Physiol Rep, 5(4).
PMID: 28235973
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Beier E et al. (2015), Heavy Metal Ion Regulation of Gene Expression: MECHANISMS BY WHICH LEAD INHIBITS OSTEOBLASTIC BONE-FORMING ACTIVITY THROUGH MODULATION OF THE Wnt/beta-CATENIN SIGNALING PATHWAY. J Biol Chem, 290(29):18216-26.
PMID: 25975268
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ryan Z et al. (2013), Sclerostin alters serum vitamin D metabolite and fibroblast growth factor 23 concentrations and the urinary excretion of calcium. Proc Natl Acad Sci U S A, 110(15):6199-204.
PMID: 23530237

Recombinant Mouse SOST/Sclerostin Protein

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Product Specifications for Recombinant Mouse SOST/Sclerostin Protein

Formulation, Preparation and Storage

Carrier Free

1589-ST

Carrier

1589-ST/CF

Carrier Free

Reconstitution Calculator

Background: SOST/Sclerostin

Citations for Recombinant Mouse SOST/Sclerostin Protein (6)

Product Documents for Recombinant Mouse SOST/Sclerostin Protein

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