Product Specifications for Recombinant Mouse SLITRK4 His-tag Protein, CF
Mouse myeloma cell line, NS0-derived mouse SLITRK4 protein Asp19-Ser618, with a C-terminal 6-His tag
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
90-100 kDa, reducing conditions
Measured by its ability to inhibit neurite outgrowth of dissociated E13 chick embryonic dorsal root ganglia (DRG) neurons. Able to significantly inhibit neurite outgrowth when immobilized as a 3 µL droplet containing 200 ng on a nitrocellulose-coated microplate
Scientific Data Examples for Recombinant Mouse SLITRK4 His-tag Protein, CF
Recombinant Mouse SLITRK4 His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse SLITRK4 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 90-100 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, ≤ -20 °C under sterile conditions after reconstitution.
SLITRK4 is an approximately 90 kDa type I transmembrane member of the SLITRK family of proteins which contain a slit-like extracellular region and a Trk-like cytoplasmic region (1). Within the extracellular domain (ECD), the mouse SLITRK4 shares 98% amino acid sequence identity with human SLITRK4. The ECD of SLITRK4 contains 6 leucine rich repeats (LRR) followed by a C-terminal LRR domain, and 6 more LRRs flanked by a pair of N- and C-terminal LRR domains (2, 3). SLITRK4 is expressed in the testis and multiple regions of the brain, particularly the cerebral cortex, cerebellum, and hippocampus (4). SLITRK4 can suppress neurite outgrowth and promote the formation of excitatory presynaptic structures (3, 5). SLITRK genes are also differentially expressed in brain tumors, including astrocytoma, oligodendroglioma, glioblastoma, medulloblastoma, and supratentorial primitive neuroectodermal tumor (PNET) (2).
Ko, J. (2012) Mol. Cells 34:335.
Aruga, J. et al. (2003) Gene 315:87.
Aruga, J. and K. Mikoshiba (2003) Mol. Cell Neurosci. 24:117.
Yim, Y.S. et al. (2013) Proc. Natl. Acad. Sci. USA 110:4057.
Takahashi, H. et al. (2012) Nat. Neurosci. 15:389.
SLIT and NTRK-like Family, Member 4
Entrez Gene IDs
139065 (Human); 245446 (Mouse)
DKFZp547M2010, SLIT and NTRK-like family, member 4, SLIT and NTRK-like protein 4, SLITRK4, slit and trk like gene 4
Citations for Recombinant Mouse SLITRK4 His-tag Protein, CF
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Product Documents for Recombinant Mouse SLITRK4 His-tag Protein, CF
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