Mouse myeloma cell line, NS0-derived mouse SIRP beta 1a protein Val28-Gly360, with a C-teminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
48-62 kDa, under reducing conditions
Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP beta 1a His-tag (Catalog # 10289-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED50 of 30-180 ng/mL.
Recombinant Mouse SIRP beta 1a His-tag Protein Binding Activity
When Recombinant Mouse SIRP beta 1a His-tag (Catalog # 10289-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED50 of 30-180 ng/mL.
Recombinant Mouse SIRP beta 1a His-tag Protein SDS-PAGE
2 μg/lane of Recombinant Mouse SIRP beta 1a His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-62 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: SIRP beta 1a
Signal-regulatory protein beta 1a (SIRP beta 1a) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Mouse SIRP beta 1a shares 57% and 59% amino acid sequence identity with human SIRP beta 1 and rat SIRP alpha, respectively. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).
vanBeek, E.M. et al. (2005) J. Immunol. 175:7781.
van den Berg, T. et al. (2008) Trends in Immunology 29:203.
Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132.
Matozaki, T. et al. (2009) Trends in Cell Biology 19:72.
Dietrich, J. et al. (2000) J Immunol. 164:9.
Brook, G. et al. (2004) J. Immunol. 173:2562.
Hayashi, A. et al. (2004) J Biol Chem. 279:29450.
Fournier, B. et al. (2012) J. Biol. Chem. 287:19386.
Seiffert, M. et al. (2001) Blood 97:2741.
Signal-regulatory Protein beta 1A
Entrez Gene IDs
SIRP beta 1a, SIRPB1A
Citations for Recombinant Mouse SIRP beta 1a His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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