Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Name: Recombinant Mouse SIRP beta 1a His-tag Protein, CF
Brand: R&D Systems
SKU: 10289-SB
Price: 428 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 10289-SB

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Key Product Details

Source:	NS0
Accession #:	NP001002898
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Product Specifications for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse SIRP beta 1a protein Val28-Gly360, with a C-teminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Val28
Predicted Molecular Mass	38 kDa
SDS-PAGE	48-62 kDa, under reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Mouse SIRP beta 1a His-tag (Catalog # 10289-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP‑D (Catalog # 1920-SP) binds with an ED₅₀ of 30-180 ng/mL.

Scientific Data Examples for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

	Recombinant Mouse SIRP beta 1a His-tag Protein Binding Activity When Recombinant Mouse SIRP beta 1a His-tag (Catalog # 10289-SB) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human SP-D (Catalog # 1920-SP) binds with an ED₅₀ of 30-180 ng/mL.
	Recombinant Mouse SIRP beta 1a His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse SIRP beta 1a His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 48-62 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10289-SB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SIRP beta 1a

Signal-regulatory protein beta 1a (SIRP beta 1a) is a disulfide-linked type I membrane glycoprotein that belongs to the SIRP/SHPS (CD172) family of the immunoglobulin (Ig) superfamily. The SIRP family are paired receptors that have similar extracellular domains but differing C-terminal domains and functions (1). Members of this family are characterized by an extracellular region containing a V-set Ig domain containing a J-like sequence and two C1-set Ig domains. Positively charged residues within the transmembrane domain mediate interactions with DAP12 proteins which contain immunoreceptor tyrosine-based activation motifs (ITAMs) (3). Proteins in the SIRP family are typically expressed in cells of monocyte, macrophage or dendritic lineages (4). Mouse SIRP beta 1a shares 57% and 59% amino acid sequence identity with human SIRP beta 1 and rat SIRP alpha, respectively. SIRP beta 1 has a relatively short cytoplasmic region and lacks the signaling motifs for association with phosphatases. However, formation of the SIRP beta 1/DAP12 complex in myeloid cells induce tyrosine phosphorylation, mitogen-activated protein kinase activation, and cellular activation (5, 6). Engagement of SIRP beta 1 by specific monoclonal antibodies promoted Fc gamma receptor-dependent or -independent phagocytosis in mouse peritoneal macrophages (7). Surfactant protein D (Sp-D) has been shown to bind SIRP alpha and SIRP beta 1 in a calcium-dependent and sugar-specific manner on a distinct binding site from CD47 (8). Although the SIRP beta 1 extracellular regions share a high degree of homology with the SIRP alpha, SIRP beta 1 has been shown not to bind CD47 (9).

Item	Value
References	vanBeek, E.M. et al. (2005) J. Immunol. 175:7781. van den Berg, T. et al. (2008) Trends in Immunology 29:203. Liu, Y. et al. (2005) Journal of Biological Chemistry 280:36132. Matozaki, T. et al. (2009) Trends in Cell Biology 19:72. Dietrich, J. et al. (2000) J Immunol. 164:9. Brook, G. et al. (2004) J. Immunol. 173:2562. Hayashi, A. et al. (2004) J Biol Chem. 279:29450. Fournier, B. et al. (2012) J. Biol. Chem. 287:19386. Seiffert, M. et al. (2001) Blood 97:2741.
Long Name	Signal-regulatory Protein beta 1A
Entrez Gene IDs	320832 (Mouse)
Alternate Names	SIRP beta 1a, SIRPB1A

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Recombinant Mouse SIRP beta 1a His-tag Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Scientific Data Examples for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: SIRP beta 1a

Citations for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

Product Documents for Recombinant Mouse SIRP beta 1a His-tag Protein, CF

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