Recombinant Mouse sFRP-4 Protein, CF

Name: Recombinant Mouse sFRP-4 Protein, CF
Brand: R&D Systems
SKU: 9806-SF

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9806-SF

Citations (2)

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Key Product Details

Source:	NS0
Accession #:	Q9Z1N6
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse sFRP-4 Protein, CF

Product Specifications for Recombinant Mouse sFRP-4 Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse sFRP-4 protein Ala22-Ser351
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala22
Predicted Molecular Mass	38 kDa
SDS-PAGE	47-63 kDa, reducing conditions
Activity	Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.2-1.2 ug/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Scientific Data Examples for Recombinant Mouse sFRP-4 Protein, CF

	Recombinant Mouse sFRP-4 Protein Bioactivity Recombinant Mouse sFRP-4 (Catalog # 9806-SF) activates Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED₅₀ for this effect is 0.2-1.2 µg/mL in the presence of 5 ng/mL Recombinant Mouse Wnt-3a (Catalog # 1324-WN).

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9806-SF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: sFRP-4

Secreted Frizzled Related Protein-4 (sFRP-4), also known as DDC-4, FrpAP, frpHE and FrzB-2, belongs to a family of Wnt-binding proteins with homology to the ligand-binding domain of the Frizzled receptors (1, 2). The sFRP proteins are approximately 30-35 kDa in size and contain an N-terminal Frizzled-like domain with 10 conserved cysteines and a Netrin-like C-terminal domain (1-3). Of all the sFRPs, sFRP-4 is most closely related to sFRP-3 (1). Mature mouse sFRP-4 shares 92% and 96% amino acid (aa) sequence identity with human and rat sFRP-4, respectively. sFRP-4 is expressed in brain, kidney, lung, ovary, prostate, mammary gland, and endometrium (1, 2). This protein shows complex functions with respect to cell survival: it is up‑regulated with apoptosis during ovulation (3), regulates apoptosis in chondrocytes (4), and promotes apoptosis in mammary glands when expressed in transgenic mice (5). On the other hand, sFRP-4 can also act to enhance growth as it is up‑regulated in endometrial and breast carcinomas (6, 7). Since it is not detected in other carcinomas such as the ovary, colon, and pancreas, its role in cancer is likely to be tissue dependent (6). In addition, sFRP-4 is characterized as a circulating phosphaturic factor expressed by tumors associated with osteomalacia that antagonizes renal Wnt signaling (8).

Item	Value
References	Jones, S.E. and C. Jomary (2002) Bioessays 24:811. Wolf, V. et al. (1997) FEBS Letters 417:385. Drake, J.M. et al. (2003) Apoptosis 8:389. James, I.E. et al. (2000) Osteoarthritis & Cartilage 8:452. Lacher, M.D. et al. (2003) Cell Death Differ. 10:528. Abu-Jawdeh, G. et al. (1999) Lab Invest. 79:439. Wong, S.C. et al. (2002) J. Pathol. 196:145. Berndt, T. et al. (2003) J. Clin. Invest. 112:785.
Long Name	Secreted Frizzled Related Protein 4
Entrez Gene IDs	6424 (Human); 20379 (Mouse); 89803 (Rat)
Alternate Names	DDC-4, FRP-4, FRP-AP, FRPHEMGC26498, Frizzled protein, human endometrium, FrzB2, frpHE, sFRP-4, sFRP4, secreted frizzled-related protein 4, secreted frizzled-related protein 4; secreted frizzled-related protein 4

Citations for Recombinant Mouse sFRP-4 Protein, CF (2)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse sFRP-4 Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

H Guo et al. (2019), Secreted Frizzled-related protein 4 inhibits the regeneration of hair follicles PeerJ, 6(0):e6153.
PMID: 30631645
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Chen et al. (2019), Sfrp4 repression of the Ror2/Jnk cascade in osteoclasts protects cortical bone from excessive endosteal resorption Proc. Natl. Acad. Sci. U.S.A., 0(0).
PMID: 31239337

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