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Recombinant Mouse Semaphorin 6C Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2108-S6

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2108-S6-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Semaphorin 6C protein
Ala26-Ile602, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala26

Predicted Molecular Mass

64.0 kDa

SDS-PAGE

74 kDa, reducing conditions

Activity

Measured by its ability to cause collapse of chick embryonic dorsal root ganglia (DRG) neuron growth cones.
Concentration of 2.5-10 μg/mL in the presence of 20 ng/mL of rhNT-3 (R&D Systems, Catalog # 267-N3) is sufficient to cause significant growth cone collapse.
Optimal concentrations should be determined by each laboratory for each application.

Formulation, Preparation and Storage

2108-S6
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Semaphorin 6C

Semaphorin 6C (Sema6C; previously Sema Y) is a 120 kDa member of the Semaphorin family of axon guidance molecules (1 - 3). The four known Class 6 semaphorins are type I transmembrane glycoproteins that are most like Class 1 invertebrate semaphorins in structure, and exhibit neuropilin-independent binding to specific plexin A receptors (1 - 3). Amino acid (aa) identity of Class 6 semaphorins is around 40% overall, but 53 - 64% within the Sema domain. Sema6C is expressed developmentally in subregions of the central and peripheral nervous systems, heart, and kidney, and primarily in skeletal muscle in adults (3, 4). Mouse Sema6C cDNA encodes 931 aa, including a 25 aa signal sequence, a 580 aa extracellular domain (ECD) including the Sema domain, a 21 aa transmembrane sequence and a 305 aa cytoplasmic portion. Alternate exon splicing creates a 923 aa short form (Sema6C.3) that is lacking aa 185 - 224 within the Sema domain, but contains 32 unique aa inserted at aa 586; postnatally, this form predominates in muscle (2, 3). The long form predominates in brain, especially in areas of increased plasticity (4). Mouse Sema6C ECD shares 98%, 92%, 92%, 92% and 88% aa identity with corresponding rat, human, porcine, equine and canine sequences, respectively. Sema6C, along with Sema6D, is co-expressed with and binds to Plexin A1 (5). This interaction is thought to guide proprioceptive peripheral neurons by repulsion, excluding them from the superficial dorsal horn of the spinal cord (5). Sema6C is downregulated and redistributed following denervation or axotomy, potentially promoting regrowth (4, 6). In muscle, Sema6C is concentrated at neuromuscular junctions (6).

References

  1. Zhou, Y. et al. (2008) Trends Biol. Sci. 33:161.
  2. Qu, X. et al. (2002) J. Biol. Chem. 277:35574.
  3. Kikuchi, K. et al. (1999) Mol. Cell. Neurosci. 13:9.
  4. Burgaya, F. et al. (2006) Mol. Cell. Neurosci. 33:321.
  5. Yoshida, Y. et al. (2006) Neuron 52:775.
  6. Svensson, A. et al. (2008) J. Mol. Hist. 39:5.

Alternate Names

Sema6C, Semaphorin Y

Entrez Gene IDs

10500 (Human); 20360 (Mouse)

Gene Symbol

SEMA6C

UniProt

Additional Semaphorin 6C Products

Product Documents for Recombinant Mouse Semaphorin 6C Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Semaphorin 6C Protein, CF

For research use only

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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