Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF

Name: Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF
Brand: R&D Systems
SKU: 8916-S8

Catalog # 8916-S8 | R&D Systems, Inc. a Bio-Techne Brand

Citations (5)

Product Datasheet / COA / SDS

Setting the standard in quality research reagents

Key Product Details

Source:	E. coli
Structure / Form:	Non-covalent heterodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF

Technical Data
Citations (5) / Reviews

Product Specifications for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF

Source

E. coli-derived mouse S100A8/S100A9 Heterodimer protein

Mouse S100A8
(Pro2-Glu89)
Accession # P27005

Mouse S100A9 (Ala2-Lys113) Accession # P31725
N-terminus	C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Pro2 (S100A8), Ala2 (S100A9)

Predicted Molecular Mass

10 kDa (S100A8), 13 kDa (S100A9)

SDS-PAGE

10 kDa (S100A8), 13 kDa (S100A9)

Activity

Measured by its ability to induce IL-6 secretion by A375 human melanoma cells. Hibino, T. et al. (2013) Cancer Res. 73:172. The ED₅₀ for this effect is 1.5-6 μg/mL.

Scientific Data Examples for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF

	Recombinant Mouse S100A8/S100A9 Heterodimer Protein Bioactivity Recombinant Mouse S100A8/S100A9 (Catalog # 8916-S8) induces IL-6 secretion by A375 human melanoma cells. The ED₅₀ for this effect is 1.5-6 μg/mL.
	Recombinant Mouse S100A8/S100A9 Heterodimer Protein SDS-PAGE 1 μg/lane of Recombinant Mouse S100A8/S100A9 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing R bands at 6.3, 10.8 kDa and NR band at 6, 9.6 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8916-S8

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution	Reconstitute at 500 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100A8/S100A9 Heterodimer

S100A8 (also known as MRP8, Calgranulin A, and CP-10) and S100A9 (also known as MRP14 and Calgranulin B) are pro-inflammatory members of the S100 family of secreted calcium binding proteins (1, 2). The 10 kDa S100A8 and 14 kDa S100A9 each contain two EF-hand calcium binding motifs. Mouse S100A8 shares 57% and 80% amino acid (aa) sequence identity with human and rat S100A8, respectively. Mouse S100A9 shares 57% and 79% amino acid sequence identity with human and rat S100A9, respectively (3, 4). S100A8 and S100A9 can associate into non-covalent homodimers and 34-35 kDa heterodimers known as S100A8/9 or Calprotectin. The presence of calcium and zinc also induces the formation of the tetramer (5, 6). S100A8 and S100A9 are up-regulated in neutrophils, monocytes, Schwann cells, and keratinocytes at sites of inflammation (7-13). The S100A8/9 heterodimer is elevated in rheumatoid arthritis synovial fluid and in the serum of cardiovascular disease, atopic dermatitis, and psoriatic arthritis patients (1, 9, 13-15). It exerts its effects through the receptors RAGE and TLR4 (11, 16). The heterodimer promotes neutrophil infiltration into sites of inflammation and inflammatory cytokine production by monocytes (5, 8, 9, 12). It promotes astrocyte proliferation (16) and the suppression of tumor growth by promoting the influx and activation of NK cells (10, 11). The S100A8/9 heterodimer additionally binds to unsaturated fatty acids such as arachidonic acid (7), and it sequesters manganese, thereby restricting the growth of Mn-dependent bacteria (17).

Item	Value
References	Averill, M.M. et al. (2012) Arterioscler. Thromb. Vasc. Biol. 32:223. Kerkhoff, C. et al. (2012) Exp. Dermatol. 21:822. Lagasse, E. and I.L. Weissman (1992) Blood 79:1907. Lackmann, M. et al. (1993) J. Immunol. 150:2981. Ryckman, C. et al. (2003) J. Immunol. 170:3233. Vogl, T. et al. (2006) Biochim. Biophys. Acta 1763:1298. Siegenthaler, G. et al. (1997) J. Biol. Chem. 272:9371. Volz, H.C. et al. (2012) Basic Res. Cardiol. 107:250. Sunahori, K. et al. (2006) Arthritis Res. Ther. 8:R69. Zhang, L. et al. (2015) Mol. Med. Rep. 11:4093. Narumi, K. et al. (2015) J. Immunol. 194:5539. Chernov, A.V. et al. (2015) J. Biol. Chem. 290:11771. Jin, S. et al. (2014) Exp. Dermatol. 23:938. Vogl, T. et al. (2014) Nat. Commun. 5:4593. Hansson, C. et al. (2014) J. Immunol. Res. 2014:696415. Ryu, M-J. et al. (2012) J. Biol. Chem. 287:22948. Damo, S.M. et al. (2013) Proc. Natl. Acad. Sci. USA 110:3841.
Long Name	S100 Calcium Binding Protein A8/S100 Calcium Binding Protein A9
Entrez Gene IDs	6279 (Human)
Alternate Names	Calprotectin, S100A8/S100A9 Heterodimer

Citations for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF (5)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse S100A8/S100A9 Heterodimer Protein, CF include:

Species: Human
Sample Types: Whole Cells
Applications: Bioassay

N Maehara et al. (2021-09-14), AIM/CD5L attenuates DAMPs in the injured brain and thereby ameliorates ischemic stroke Cell Reports, 2021-036(11):109693.
PMID: 34525359
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

M Wu et al. (2018-09-25), S100A8/A9 induces microglia activation and promotes the apoptosis of oligodendrocyte precursor cells by activating the NF-?B signaling pathway Brain Res. Bull., 2018-00(0).
PMID: 30266587
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

H Gong et al. (2018-09-04), Hippocampal Mrp8/14 signaling plays a critical role in the manifestation of depressive-like behaviors in mice J Neuroinflammation, 2018-015(1):252.
PMID: 30180864
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

A Basu et al. (2018-04-18), A Novel S100A8/A9 Induced Fingerprint of Mesenchymal Stem Cells associated with Enhanced Wound Healing Sci Rep, 2018-08(1):6205.
PMID: 29670130
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

S Son et al. (2017-10-19), Advanced glycation end products impair NLRP3 inflammasome-mediated innate immune responses in macrophages J. Biol. Chem., 2017-10(0).
PMID: 29051224

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