Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Name: Recombinant Mouse ROBO2 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 8366-RB
Price: 438 USD

Catalog # 8366-RB | R&D Systems, Inc. a Bio-Techne Brand

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	NP_780758
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse ROBO2 protein

Mouse ROBO2 (Ser22-Ala863) Accession # NP_780758	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE with silver staining

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser22

Predicted Molecular Mass

120 kDa

SDS-PAGE

100-155 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When recombinant human Slit3 or Recombinant Mouse Slit3 (Catalog # 3629-SL) is coated at 0.25 μg/mL, Recombinant Mouse ROBO2 Fc Chimera binds with an apparent K_d <2 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

8366-RB

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ROBO2

ROBO2 is a type I transmembrane glycoprotein that is part of the ROBO family of guidance molecules. Mouse ROBO2 is synthesized as a 1470 amino acid (aa) precursor and has a predicted molecular weight of approximately 160 kDa. Its extracellular domain contains five C2-type Ig-like domains and three fibronectin type III domains, and shares 98% aa sequence identity with the human and rat orthologs. Alternative splicing of mouse Robo2 creates an 820 aa isoform that contains a 14 aa substitution for aa 807-946 and then terminates after this substitution. A 1527 aa isoform, termed ROBO2a, has also been identified (1). ROBO2a contains a longer signal peptide and is cleaved at a different position resulting in four extra aa in its N-terminus. It also contains an additional 42 aa that is inserted after aa 1049 (1). Mouse ROBO2 is ubiquitously expressed in embryonic and adult tissues, with the highest expression levels in the adult being seen in the brain, liver, and kidney (1-9). Mouse ROBO2a is also ubiquitously expressed during development; however, it is only expressed in the brain in adults (1). ROBO2 acts as a receptor for the Slit family of chemorepulsion molecules and facilitates Slit-mediated growth cone repulsion (5). It is highly expressed on axons that do not cross the ventral midline of the nervous system and on axons that have already crossed (9-11). As a result, Slit/ROBO2 signaling regulates guidance and midline crossing of growing axons (3, 5, 11-16). Slit/ROBO2 signaling has also been shown to mediate repulsion of neuronal cell bodies away from the floor plate during development (12). Furthermore, interactions between Slit and ROBO2 have also been shown to be critical for development of the foregut, lungs, kidney, and heart (6-8, 17, 18).

Item	Value
References	Yue, Y. et al. (2006) Genomics 88:772. Piper, M. et al. (2000) Mech. Dev. 94:213. Erskine, L. et al. (2000) J. Neurosci. 20:4975. Vargesson, N. et al. (2001) Mech. Dev. 106:175. Guthrie, S. (2001) Curr. Biol. 11:R300. Greenberg, J.M. et al. (2004) Dev. Dyn. 230:350. Medioni, C. et al. (2010) Dev. Dyn. 239:3303. Domyan, E.T. et al. (2013) Dev. Cell 24:52. Sundaresan, V. et al. (2004) J. Comp. Neurol. 468:467. Rajagopalan, S. et al. (2000) Neuron 28:767. Mambetisaeva, E.T. et al. (2005) Dev. Dyn. 233:41. Kim, M. et al. (2011) Dev. Biol. 358:181. Fouquet, C. et al. (2007) J. Neurosci. 27:3037. López-Bendito, G. et al. (2007) J. Neurosci. 27:3395. Goldberg, D. et al. (2013) Dev. Dyn. 242:9. Wang, S. et al. (2013) J. Neurosci. 33:12242. Grieshammer, U. et al. (2004) Dev. Cell 6:709. Mommersteeg, M.T. et al. (2013) Circ. Res. 112:465.
Long Name	Roundabout Homolog 2
Entrez Gene IDs	6092 (Human); 268902 (Mouse); 84409 (Rat)
Alternate Names	Drosophila) homolog 2, ROBO2, SAX3, roundabout, axon guidance receptor, homolog 2 (Drosophila)

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Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Catalog # 8366-RB | R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: ROBO2

Citations for Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Product Documents for Recombinant Mouse ROBO2 Fc Chimera Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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