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Recombinant Mouse RGM-A Protein, CF

Bio-Techne includes R&D Systems | Catalog # 2458-RG

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2458-RG-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse RGM-A protein
Mouse RGM-A
Cys48-Gly421 &
Pro170-Gly421
Accession # Q6PCX7
DI 6-His tag
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys48 (proprotein) & Pro170 (mature)

Predicted Molecular Mass

42.5 kDa

SDS-PAGE

Multiple bands between 25-37 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Human BMP-4 (Catalog # 314-BP) is coated at 1 μg/mL (100 μL/well), Recombinant Mouse RGM-A binds with an apparent Kd <50 nM.

Formulation, Preparation and Storage

2458-RG
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with 5% Trehalose.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: RGM-A

Mouse repulsive guidance molecule (RGM‑A) is a 33 kDa GPI-linked member of an expanding RGM‑related family of neuronal and muscle-expressed membrane proteins (1, 2). It is synthesized as a 454 amino acid (aa) preproprotein that contains a 47 aa signal sequence, a 122 aa N-terminal prosegment, a 258 mature region (aa 170-427) and a 27 aa C-terminal prosegment (3, 4). The N-terminal prosegment contains an RGD tripeptide and the molecule's only two potential N‑linked glycosylation sites. The mature segment shows an abbreviated von Willebrand factor domain. Proteolytic processing occurs at an aspartic acid‑proline bond, creating a predicted 32 kDa mature region (3). There is one potential alternative start site at Met17. The mature region of mouse RGM-A is 93% and 87% aa identical to human and chick mature region RGM-A, respectively. Recognizing that RGM-A possesses a cleavage site between Asp169Pro170, it is suggested that membrane-expressed RGM-A may exist as a covalent heterodimer that links a GPI-anchored mature molecule with its prosegment (1, 4, 5). When compared to mouse RGM-B and C, the mature region of mouse RGM-A shows 77% and 76% aa identity, respectively (5, 6). Cells normally described as expressing RGM-A include neurons and oligodendroglia. But this select group is expanded to endothelium, microglia and leukocytes in areas of neuronal injury (7). Functionally, recombinant chick RGM has been reported to induce collapse of temporal but not nasal growth cones, and to repel temporal retinal axons in vitro. This suggests a role in the development of the retina-superior colliculus connection. In mice, RGM-A also induces growth cone collapse on primary DRG neurons (8). And in a mouse culture system, chick RGM-A is reported to be responsible for the layered segmentation of entorhinal cortical projections to the hippocampus (9). RGM reportedly binds to neogenin and BMP-2/4, with the latter binding occuring within the context of a BMPRI:ActRIIA:RGM-A receptor complex (10-13).

References

  1. Severyn, C.J. et al. (2009) Biochem. J. 422:393.
  2. Samad, T.A. et al. (2004) J. Neurosci. 24:2027.
  3. Schmidtmer, J. et al. (2004) Gene Expr. Patterns 4:105.
  4. Matsunaga, E. & A. Chedotal (2004) Develop. Growth Differ. 46:481.
  5. Monnier, P.P. et al. (2002) Nature 419:392.
  6. Niederkofler V. et al. (2004) J. Neurosci. 24:808.
  7. Hata, K. et al. (2006) J. Cell Biol. 173:47.
  8. Conrad, S. et al. (2007) J. Biol. Chem. 282:16423.
  9. Brinks, H. et al. (2004) J. Neurosci. 24:3862.
  10. Rajagopalan S. et al. (2004) Nat. Cell Biol. 6:756.
  11. Matsunaga E. et al. (2004) Nat. Cell Biol. 6:749.
  12. Babitt, J.L. et al. (2005) J. Biol. Chem. 280:29820.
  13. Xia, Y. et al. (2007) J. Biol. Chem. 282:18129.

Long Name

Repulsive Guidance Molecule A

Alternate Names

RGMA

Entrez Gene IDs

56963 (Human); 244058 (Mouse)

Gene Symbol

RGMA

UniProt

Additional RGM-A Products

Product Documents for Recombinant Mouse RGM-A Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse RGM-A Protein, CF

For research use only

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