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Recombinant Mouse Resistin Protein

Catalog # 1069-RN | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
1069-RN-050/CF

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With Carrier
1069-RN-050

Key Product Details

Source

E. coli

Accession #

Structure / Form

Monomer and disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse Resistin protein
Ser21-Ser114

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

10 kDa (monomer)

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse Resistin is used at 0.5 μg/mL, it binds to Biotinylated Recombinant Mouse Leptin with an ED50 of 50-300 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 1069-RN
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 1069-RN/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Resistin

Resistin, also known as adipocyte-specific secretory factor (ADSF) and found in inflammatory zone 3 (FIZZ3), is a member of a family of secreted cysteine-rich peptide hormones that also includes Resistin-like molecules RELM alpha, beta, and gamma. These molecules play important roles in inflammation, glucose metabolism, and insulin resistance (1, 2). Mature mouse Resistin is a 12 kDa protein with an N-terminal alpha-helical domain and a C-terminal beta-sandwich domain that is stabilized by multiple intrachain disulfide bonds (3). Resistin circulates as noncovalent trimers and disulfide-linked hexamers, with the trimeric form showing greater bioactivity (3, 4). Resistin can also form multimers with RELM beta (4). Mature mouse Resistin shares 56% and 72% amino acid (aa) sequence identity with human and rat Resistin, respectively. It shares 34% - 42% aa sequence identity with mouse RELM alpha, beta, and gamma. In rodents, Resistin is expressed by adipocytes and in the pituitary and arcuate nucleus of the hypothalamus (5 - 9). It is upregulated during adipogenesis, in obesity, and by insulin or a high carbohydrate diet (5, 6). This is in contrast to human Resistin which is produced by macrophages and monocytes but not by adipocytes (9 - 12). Mouse Resistin induces proinflammatory molecule production in adipocytes and promotes hepatic gluconeogenesis and insulin resistance (5, 7). Human Resistin promotes lipolysis by human and mouse adipocytes, but mouse Resistin does not promote lipolysis by adipocytes of either species (10). Both mouse and human Resistin promote vascular endothelial cell sprouting in vitro and inflammatory reactions in vivo (13 - 16).

References

  1. Adeghate, E. (2004) Cell. Mol. Life Sci. 61:2485.
  2. Asano, T. et al. (2006) Curr. Diabetes Rev. 2:449.
  3. Patel, S.D. et al. (2004) Science 304:1154.
  4. Chen, J. et al. (2002) J. Endocrinol. 175:499.
  5. Steppan, C.M. et al. (2001) Nature 409:307.
  6. Kim, K-H. et al. (2001) J. Biol. Chem. 276:11252.
  7. Banerjee, R.R. et al. (2004) Science 303:1195.
  8. Rajala, M.W. et al. (2002) Mol. Endocrinol. 16:1920.
  9. Morash, B.A. et al. (2002) FEBS Lett. 526:26.
  10. Ort, T. et al. (2005) Endocrinology 146:2200.
  11. Nagaev, I. et al. (2006) Plos ONE 1:e31.
  12. Savage, D.B. et al. (2001) Diabetes 50:2199.
  13. Mu, H. et al. (2006) Cardiovasc. Res. 70:146.
  14. Robertson, S.A. et al. (2008) Regul. Pept. August 5 epub.
  15. Bokarewa, M. et al. (2005) J. Immunol. 174:5789.
  16. Burnett, M.S. et al. (2005) Atherosclerosis 182:241.

Alternate Names

ADSF, FIZZ3, HXCP1, RETN

Entrez Gene IDs

56729 (Human); 57264 (Mouse)

Gene Symbol

RETN

UniProt

Product Documents for Recombinant Mouse Resistin Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Resistin Protein

For research use only

Citations for Recombinant Mouse Resistin Protein (2)

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