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Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

Catalog # 10333-TK | R&D Systems, Inc. a Bio-Techne Brand
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10333-TK-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PTK7/CCK4 protein
Mouse PTK7/CCK4
(Ala23-Thr696)
Accession # Q8BKG3
IEGRMDP Mouse IgG2A
(Glu98-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala23

Predicted Molecular Mass

102 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324). The ED50 for this effect is 8-48 μg/mL.

Scientific Data Images for Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

Recombinant Mouse PTK7/CCK4 Fc Chimera Protein Binding Activity

Recombinant Mouse PTK7/CCK4 Fc Chimera Protein Binding Activity

Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324) . The ED50 for this effect is 8-48 µg/mL.
Recombinant Mouse PTK7/CCK4 Fc Chimera Protein SDS-PAGE

Recombinant Mouse PTK7/CCK4 Fc Chimera Protein SDS-PAGE

2 μg/lane of Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa and 220-160 kDa, respectively.

Formulation, Preparation and Storage

10333-TK
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PTK7/CCK4

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a member of the receptor tyrosine kinase superfamily (1, 2). PTK7 is a type I transmembrane receptor with a large extracellular domain (ECD) containing 7 Ig-like C2 type loops, a transmembrane domain, and a cytoplasmic tyrosine kinase homology domain. However, due to the lack of the DFG triplet motif required for catalytic activity in the kinase domain, PTK7 is considered a pseudokinase (1, 3). The mature ECD of mouse PTK7 shares 92% amino acid sequence identity with human PTK7. PTK7 is expressed in a wide array of tissue types ranging from lung and liver to kidney and placenta and has been linked to a broad range of functions (5). While originally identified as being over-expressed in colon carcinomas, PTK7 has been shown to play a role in embryogenesis, epithelial tissue organization, angiogenesis, cell motility, and survival (1, 6, 7). PTK7 has been shown to be an important regulator of the Wnt signaling pathways, both canonical and non-canonical, and is linked to the regulation of the planar cell polarity pathway (3, 6). Proteolysis of full-length PTK7 by MMPs (matrix metalloproteinases), ADAMs (a disintegrin domain and metalloproteinases), and gamma-secretases results in a soluble N-terminal fragment and several C-terminal, membrane-associated or intracellular proteolytic fragments (3, 6). The soluble form is a co-receptor for the Semaphorin/Plexin and VEGF signaling pathways (8). Deregulation of PTK7 signaling has now been observed in numerous cancers including colon, gastric, lung, and acute myeloid leukemia (1, 3). Recent studies have shown that PTK7 expression promoted increased migration and resistance to apoptosis in leukemic cells and acute myeloid leukemia (AML) blasts, while knock-down of PTK7 induced apoptosis in colorectal carcinoma cells (2, 7). Further, other studies have suggested that the ratio of full-length vs. cleaved protein, not just expression, contributes to PTK7's metastatic effects in cancer (6).

References

  1. Shin, W. et al. (2008) Biochem. Bioph. Res. Co. 371:793.
  2. Meng, L. et al. (2010) PLoS ONE 5:e14018.
  3. Golubkov, V. et al. (2010) J. Biol. Chem. 285:35740.
  4. Berger, H. et al. (2017) Front. Cell Dev. Biol. 5:31.
  5. Park, S. et al. (1996) J. Biochem. 119:235.
  6. Golubkov, V. et al. (2014) J. Biol. Chem. 289:24238.
  7. Prebet, T. et al. (2010) Blood. 116:2315.
  8. Peradziryi, H. et al. (2012) Arch. Biochem. Biophys. 524:71.

Long Name

Protein Tyrosine Kinase 7

Alternate Names

CCK4

Entrez Gene IDs

5754 (Human); 71461 (Mouse); 301242 (Rat)

Gene Symbol

PTK7

UniProt

Additional PTK7/CCK4 Products

Product Documents for Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

For research use only

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