Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

Name: Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10333-TK

Catalog # 10333-TK

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	Q8BKG3
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse PTK7/CCK4 protein

Mouse PTK7/CCK4 (Ala23-Thr696) Accession # Q8BKG3	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala23

Predicted Molecular Mass

102 kDa

SDS-PAGE

110-130 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324). The ED₅₀ for this effect is 8-48 μg/mL.

Recombinant Mouse PTK7/CCK4 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse PTK7/CCK4 Fc Chimera Protein Binding Activity Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) binds Biotinylated Recombinant Mouse Wnt-3a (Catalog # BT1324). The ED₅₀ for this effect is 8-48 µg/mL.
	Recombinant Mouse PTK7/CCK4 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse PTK7/CCK4 Fc Chimera (Catalog # 10333-TK) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 110-130 kDa and 220-160 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10333-TK

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: PTK7/CCK4

Protein tyrosine kinase 7 (PTK7), also known as colon carcinoma kinase 4 (CCK4), is a member of the receptor tyrosine kinase superfamily (1, 2). PTK7 is a type I transmembrane receptor with a large extracellular domain (ECD) containing 7 Ig-like C2 type loops, a transmembrane domain, and a cytoplasmic tyrosine kinase homology domain. However, due to the lack of the DFG triplet motif required for catalytic activity in the kinase domain, PTK7 is considered a pseudokinase (1, 3). The mature ECD of mouse PTK7 shares 92% amino acid sequence identity with human PTK7. PTK7 is expressed in a wide array of tissue types ranging from lung and liver to kidney and placenta and has been linked to a broad range of functions (5). While originally identified as being over-expressed in colon carcinomas, PTK7 has been shown to play a role in embryogenesis, epithelial tissue organization, angiogenesis, cell motility, and survival (1, 6, 7). PTK7 has been shown to be an important regulator of the Wnt signaling pathways, both canonical and non-canonical, and is linked to the regulation of the planar cell polarity pathway (3, 6). Proteolysis of full-length PTK7 by MMPs (matrix metalloproteinases), ADAMs (a disintegrin domain and metalloproteinases), and gamma-secretases results in a soluble N-terminal fragment and several C-terminal, membrane-associated or intracellular proteolytic fragments (3, 6). The soluble form is a co-receptor for the Semaphorin/Plexin and VEGF signaling pathways (8). Deregulation of PTK7 signaling has now been observed in numerous cancers including colon, gastric, lung, and acute myeloid leukemia (1, 3). Recent studies have shown that PTK7 expression promoted increased migration and resistance to apoptosis in leukemic cells and acute myeloid leukemia (AML) blasts, while knock-down of PTK7 induced apoptosis in colorectal carcinoma cells (2, 7). Further, other studies have suggested that the ratio of full-length vs. cleaved protein, not just expression, contributes to PTK7's metastatic effects in cancer (6).

Item	Value
References	Shin, W. et al. (2008) Biochem. Bioph. Res. Co. 371:793. Meng, L. et al. (2010) PLoS ONE 5:e14018. Golubkov, V. et al. (2010) J. Biol. Chem. 285:35740. Berger, H. et al. (2017) Front. Cell Dev. Biol. 5:31. Park, S. et al. (1996) J. Biochem. 119:235. Golubkov, V. et al. (2014) J. Biol. Chem. 289:24238. Prebet, T. et al. (2010) Blood. 116:2315. Peradziryi, H. et al. (2012) Arch. Biochem. Biophys. 524:71.
Long Name	Protein Tyrosine Kinase 7
Entrez Gene IDs	5754 (Human); 71461 (Mouse); 301242 (Rat)
Alternate Names	CCK-4, CCK4, CCK4Colon carcinoma kinase 4, PTK7, PTK7 protein tyrosine kinase 7, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7, colon carcinoma kinase-4

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