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Recombinant Mouse Prolactin R Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1309-PR

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1309-PR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Prolactin R protein
Mouse Prolactin R
(Ser21 - Asp229)
Accession # Q08501
DIEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.1 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ser21

Predicted Molecular Mass

51 kDa (monomer)

SDS-PAGE

60-68 kDa, reducing conditions

Activity

Measured by its ability to inhibit Prolactin-induced proliferation of Nb2‑11 rat lymphoma cells. Gout, P.W. et al. (1980) Cancer Res. 40:2433.
The ED50 for this effect is 1-5 ng/mL in the presence of 0.5 ng/mL of rhProlactin.

Formulation, Preparation and Storage

1309-PR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Prolactin R

The neuroendocrine pituitary hormone Prolactin (PRL), also known as lactotrophin, mamotrophin, luteotropic hormone (LTH), or luteotropin, is a secreted hormone that affects reproduction and homeostasis in vertebrates. The functions of PRL can be placed in six broad categories: 1) reproduction and lactation; 2) growth and development; 3) endocrinology and metabolism; 4) brain and behavior; 5) immunomodulation; and 6) electrolyte balance (1, 2). PRL is secreted by the anterior pituitary gland, mammary gland, placenta, brain, uterus, decidua, dermal fibroblasts, B cells, T cells, NK cells, and some breast cancer cell lines. Although the major form of PRL is a 23 kDa monomeric protein, splice variants of 14, 16, and 22 kDa have been identified. PRL has also been found to be glycosylated, phosphorylated, dimerized, and polymerized. Glycosylation, phosphorylation, dimerization, or polymerization of PRL result in lower activity (2).

A single chain membrane-bound protein belonging to the class 1 cytokine superfamily mediates cell activation by PRL. The PRL receptor (PRL R) contains extracellular, transmembrane, and intracellular domains. Transcriptional regulation of the PRL R gene results in several different species-dependent isoforms of PRL R being produced. Although the cytoplasmic domains of the different isoforms vary in length and composition, their extracellular domains are identical. In mice, one long and three short forms of PRL receptor have been described (2). PRL receptors are found in mammary tissue, pituitary gland, brain, heart, lung thymus, spleen, liver, pancreas, kidney, adrenal gland, uterus, skeletal muscle and skin (3). A soluble form of PRL R containing the 206 NH2-terminal amino acids of the extracellular domain is secreted by mammary epithelial cells and is found in milk. Studies of PRL receptor deficient mice have demonstrated that PRL receptor signaling is required for female fertility and mammary development. Both male and female PRL receptor deficient mice have reduced bone formation and reduced abdominal fat deposits (1).

References

  1. Kelly, P.A. et al. (2001) Biochem. Society Transaction 29:48.
  2. Freeman, M.E. et al. (2000) Physiol. Rev. 80:1532.
  3. Nagano, M. and P.A. Kelly (1994) J. Biol. Chem. 269:1337.

Long Name

Prolactin Receptor

Alternate Names

PRLR, ProlactinR

Entrez Gene IDs

5618 (Human); 19116 (Mouse); 24684 (Rat)

Gene Symbol

PRLR

UniProt

Additional Prolactin R Products

Product Documents for Recombinant Mouse Prolactin R Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Prolactin R Fc Chimera Protein, CF

For research use only

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