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Recombinant Mouse PRDC/GREM2 Protein

Bio-Techne includes R&D Systems | Catalog # 2069-PR

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Carrier Free
2069-PR-050/CF

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With Carrier
2069-PR-050

Key Product Details

Source

E. coli

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse PRDC/GREM2 protein
Arg22-Gln168, with an N-terminal Met

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

17.1 kDa (monomer)

Activity

Measured by its ability to inhibit BMP-4-induced activity in MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is 0.03-0.12 µg/mL in the presence of 50 ng/mL of rhBMP-4.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2069-PR
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2069-PR/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: PRDC/GREM2

PRDC (protein related to DAN and Cerberus) is a secreted cysteine knot-containing BMP antagonist belonging to the Cerberus/DAN (CAN) family. Mammalian CAN family members, including Gremlin, Dan, Cerberus, COCO, SOST, and USAG-1, have the conserved 6 cysteine residues that form a cysteine knot, and two additional cysteine residues located in the loops of the cysteine knot, which form an additional intrasubunit disulfide bond (1, 2). Some members of this family, including PRDC, have an additional cysteine residue used for dimerization (1, 2). Of all the CAN family members, PRDC is most closely related to Gremlin, displaying 52% amino acid sequence identity. PRDC was first identified in a screen for developmentally regulated genes by gene trapping in embryonic stem cells (3). PRDC expression is detected by in situ hybridization in the dorsal edge of the spinal cord at E10.5, in commissural neurons in the caudal part of the spinal cord two days later (3), and in the granulosa cells of selective ovarian follicles (4). In the adult, abundant levels of PRDC are detected by RT-PCR in the mouse ovary, brain, and spleen, and to a lesser degree in the colon, kidney, lung, liver, and uterus (4). PRDC acts as a specific BMP antagonist, binding to and blocking signaling induced by BMP-2 or -4, but not Activin or TGF-beta (4). Thus, PRDC expression in the ovary could be involved in follicular development by antagonizing the inhibitory effects of BMPs on FSH stimulation of progesterone (4).

References

  1. Pearce, J. et al. (1999) Dev. Biol. 209:99.
  2. Avsian-Kretchmer, O. and A. Hsueh (2004) Molecular Endocrinology 18:1.
  3. Minabe-Saegusa, C. et al. (1998) Develop. Growth Differ. 40:343.
  4. Sudo, S. et al. (2004) Jour. Biol. Chem. 279:23134.

Long Name

Protein Related to DAN and Cerberus/Gremlin-2

Alternate Names

CKTSF1B2, DAND3, GREM2

Entrez Gene IDs

64388 (Human); 23893 (Mouse)

Gene Symbol

GREM2

UniProt

Additional PRDC/GREM2 Products

Product Documents for Recombinant Mouse PRDC/GREM2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse PRDC/GREM2 Protein

For research use only

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