Product Specifications for Recombinant Mouse PIR-B Protein, CF
Mouse myeloma cell line, NS0-derived mouse PIR-B protein Gly24-Gly635, with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
85-95 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. When biotinylated recombinant rat MAG/Siglec-4a Fc Chimera is catured on Streptavidin coated plate (Catalog # CP003), Recombinant Mouse PIR-B binds with an apparent Kd < 100 nM.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with trehalose
Reconstitute at 250 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Paired immunoglobulin‑like receptor B (PIR‑B) is a transmembrane protein that acts as a negative regulator of immune responses. It is the rodent homolog of human LILRB1‑5 family proteins. It is most closely related to LILRB3 which is also known as ILT5, LIR‑3, and CD85a. Like LILRB family members, PIR‑B contains cytoplasmic immunoreceptor tyrosine‑based inhibitory motifs (ITIMs) that inhibit signaling events via phosphatase SHP‑1. PIR‑A and other LILRA family members instead contain cytoplasmic ITAMs which augment immune responses (1). Mature mouse PIR‑B is a 120 kDa glycoprotein that consists of a 618 amino acid (aa) extracellular domain (ECD) with six Ig‑like domains, a 21 aa transmembrane segment, and a 178 aa cytoplasmic domain with four ITIMs (2). The 85‑95 kDa human ILT5 contains four Ig‑like domains. Within common regions of their ECDs, mouse PIR‑B shares 73% and 55% aa sequence identity with rat PIR‑B and human ILT5, respectively. PIR‑B is expressed on dendritic cells, monocytes, macrophages, B cells, mast cells, neutrophils, eosinophils, basophils, osteoclasts, and cerebellar granule neurons (3‑10). Triggering of PIR‑B inhibits the activation of macrophages, mast cells, neutrophils, basophils, and B cells (5‑7, 9). PIR‑B also inhibits the differentiation of osteoclasts and pro‑inflammatory M1 macrophages (4, 11). PIR‑B interacts in cis and in trans with MHC I molecules to limit autoreactive humoral and cellular immune responses (12‑14). It suppresses anti‑bacterial inflammatory responses by blocking TLR9‑dependent B cell activation (14). In the CNS, PIR‑B functions as a receptor for the myelin proteins Nogo, MAG, and OMgp and mediates their inhibitory action on neurite outgrowth and axon regeneration (10). Upon binding to MAG, PIR‑B associates with TrkB and NGF R/p75 in cerebellar granule neurons (15).
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Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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