What is the half-life of this BMP protein?

Name: Recombinant Mouse Nodal Protein
Brand: R&D Systems
SKU: 1315-ND
Price: 460 USD

R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Mouse Nodal Protein

Catalog # 1315-ND | R&D Systems, Inc. a Bio-Techne Brand

Citations (12)

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Key Product Details

Source:	E. coli
Accession #:	P43021
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Nodal Protein

Product Specifications for Recombinant Mouse Nodal Protein

Source	E. coli-derived mouse Nodal protein His245-Leu354, with an N-terminal Met
Purity	>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Met
Predicted Molecular Mass	13 kDa (monomer)
Activity	Measured by its ability to induce Smad2 phosphorylation in P19 mouse embryonal carcinoma cells. Yeo, C. et al. (2001) Mol. Cell. 7:949. Approximately 100 ng/mL of Recombinant Mouse Nodal can effictively induce Smad2 phosphorylation.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

1315-ND

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution	Reconstitute at 50 μg/mL in sterile 1 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

1315-ND/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Nodal

Nodal is a secreted protein that is a member of the Transforming Growth Factor-beta (TGF-beta ) superfamily. Nodal was named for its localized expression in the mouse node during gastrulation, and is first detected in the early primitive streak during mesoderm formation. Expression of the Nodal gene occurs asymmetrically in the left, but not right, lateral plate during somitogenesis. Nodal proteins play crucial roles in mesoderm formation and both anterior-posterior and left-right axis formation during vertebrate development. Members of the Nodal gene family include mouse Nodal, chick cNR-1, frog Xnr1-4, and zebrafish cyclops. Biologically active Nodal is a disulfide-linked homodimer and contains all seven of the cysteine residues necessary for formation of the “cysteine knot” characteristic of TGF-beta -related molecules. Mouse Nodal is 34 - 39% homologous in the conserved region to other TGF-beta superfamily members. Nodal has been shown to signal through a mechanism related to the Activin pathway, and signaling is mediated through both Smad2 and 3. Nodal signaling utilizes type II Activin receptors, together with ALK4/ActRIB, or the orphan type I receptor ALK7. Nodal interacts extracellularly with members of other protein families, including Cerberus, Lefty, and EGF-CFC ligands, such as Cripto. While the Cerberus and Lefty families act as Nodal antagonists, the EGF-CFC molecules act as co-receptors to facilitate Nodal signaling. The resulting concert of regulated Nodal activity allows for the precise control of mesoderm formation, neural patterning, and axis positioning and patterning during early vertebrate development.

Item	Value
References	Kumar, A. et al. (2001) J. Biol. Chem. 276:656. Reissmann, E. et al. (2001) Genes & Dev. 15: 2010. Schier, A. and M. Shen (1999) Nature 403:385. Zhou, X. et al. (1993) Nature 361:543.
Entrez Gene IDs	4838 (Human); 18119 (Mouse)
Alternate Names	BMP-16, MGC138230, Nodal, nodal homolog, nodal homolog (mouse), nodal, mouse, homolog

Citations for Recombinant Mouse Nodal Protein (12)

Showing 1 - 10 of 12 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Nodal Protein include:

Species: Xenopus
Sample Types: In Vivo
Applications: In Vivo

P Scerbo et al. (2017-06-27), Lineage commitment of embryonic cells involves MEK1-dependent clearance of pluripotency regulator Ventx2 Elife, 2017-06(0).
PMID: 28654420
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo

Dubrulle et al. (2015-04-14), Response to Nodal morphogen gradient is determined by the kinetics of target gene induction. Elife, 2015-04(0).
PMID: 25869585
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jahn S et al. (2014-06-13), Signaling mechanisms that suppress the cytostatic actions of rapamycin. PLoS ONE, 2014-09(6):e99927.
PMID: 24927123
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Fuller K et al. (2014-04-26), Rab23 regulates Nodal signaling in vertebrate left-right patterning independently of the Hedgehog pathway. Dev Biol, 2014-0391(2):182-95.
PMID: 24780629
Species: Zebrafish
Sample Types: In Vivo
Applications: In Vivo

Kumari et al. (2013-09-10), An essential role for maternal control of Nodal signaling. Elife, 2013-02(0):e00683.
PMID: 24040511
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Souquet B et al. (2012-03-06), Nodal signaling regulates the entry into meiosis in fetal germ cells. Endocrinology, 2012-0153(5):2466-73.
PMID: 22396454
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Hansson M et al. (2009-04-07), A late requirement for Wnt and FGF signaling during activin-induced formation of foregut endoderm from mouse embryonic stem cells. Dev. Biol., 2009-0330(2):286-304.
PMID: 19358838
Species: Human, Mouse
Sample Types: Whole Cells
Applications: Bioassay

Borowiak M et al. (2009-04-03), Small molecules efficiently direct endodermal differentiation of mouse and human embryonic stem cells. Cell Stem Cell, 2009-04(4):348-58.
PMID: 19341624
Species: Xenopus
Sample Types: Whole Cells
Applications: Bioassay

Levine AJ et al. (2008-09-18), GDF3 is a BMP inhibitor that can activate Nodal signaling only at very high doses. Dev. Biol., 2008-0325(1):43-8.
PMID: 18823971
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Oki S et al. (2007-10-03), Sulfated glycosaminoglycans are necessary for Nodal signal transmission from the node to the left lateral plate in the mouse embryo. Development, 2007-1134(21):3893-904.
PMID: 17913787
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lindsley RC et al. (2006-08-30), Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 2006-0133(19):3787-96.
PMID: 16943279
Species: Rat
Sample Types: N/A, Whole Cells
Applications: Antibody Absorption, Bioassay

Wang H et al. (2006-05-18), Role and regulation of nodal/activin receptor-like kinase 7 signaling pathway in the control of ovarian follicular atresia. Mol. Endocrinol., 2006-020(10):2469-82.
PMID: 16709598

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Showing 1 - 10 of 12 citations Showing All

FAQs for Recombinant Mouse Nodal Protein

What is the half-life of this BMP protein?

R&D Systems has not determined the half-life of this recombinant human BMP protein. Assay conditions for each protein will need to be optimized by each researcher.

Recombinant Mouse Nodal Protein

Catalog # 1315-ND | R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

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Product Specifications for Recombinant Mouse Nodal Protein

Formulation, Preparation and Storage

Carrier Free

1315-ND

Carrier

1315-ND/CF

Carrier Free

Reconstitution Calculator

Background: Nodal

Citations for Recombinant Mouse Nodal Protein (12)

FAQs for Recombinant Mouse Nodal Protein

Product Documents for Recombinant Mouse Nodal Protein

Product Datasheets

COA

Certificate of Analysis

SDS

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