Product Specifications for Recombinant Mouse MGL2/CD301b Protein, CF
Mouse myeloma cell line, NS0-derived mouse MGL2/CD301b protein Ser72-Pro332, with an N-terminal 10-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
36-38 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. Immobilized rmMGL2 at 2.5 µg/mL (100 µL/well) on a Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050) coated plate can bind biotinylated beta -Galactosamine-N-Acetyl-Polyacrylamide ( beta -Gal-NAc-PAA) with a linear range of 2-150 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse Macrophage Galactose N-acetyl-Galactosamine (GalNAc) specific Lectin 2 (MGL2), also known as CD301b, is a 38 kDa member of the C-type lectin family (1). Two MGL proteins are encoded by separate genes in the mouse, but share 91% amino acid (aa) identity in the extracellular domain (ECD) (1). Only one MGL occurs in human and rat and this MGL is structurally more similar to mouse MGL1 than MGL2. However, human MGL and mouse MGL2 both bind specifically to terminal GalNAc residues, in contrast with mouse MGL1 which binds Lewis X (1, 2). GalNAc recognition is likely to be important in dendritic cell-mediated tolerance to self-gangliosides as well as recognition of tumor antigens and parasite glycoproteins (2). Both mouse MGL proteins are expressed on immature dendritic cells. Mouse MGL2 and MGL1 are markers for connective tissue macrophages of a type termed alternately activated macrophages. These macrophages are induced by IL-4 that is produced during Th2-mediated inflammatory responses to parasitic infections or allergic airway inflammation (3, 4). Quantitative RT-PCR after helminth infection shows increasing expression of MGL2 for at least 29 days while MGL1 shows a peak expression at 7 days (3). This, and data from MGL1 knockout mice,(5) indicates that MGL1 is critical during formulation of granulation tissue, but MGL2 remains involved during chronic infection. Mouse MGL2 is synthesized as a 332 aa type II transmembrane protein with an N-terminal 51 aa cytoplasmic region, a 26 aa TM segment, and a 255 aa ECD. The ECD contains one 150 aa carbohydrate recognition domain (CRD) that shows 76% and 68% aa identity with rat or human MGL, respectively.
Tsuiji, M. et al. (2002) J. Biol. Chem. 277:28892.
Van Vliet, S. J. et al. (2005) Int. Immunol. 17:661.
Citations for Recombinant Mouse MGL2/CD301b Protein, CF
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FAQs for Recombinant Mouse MGL2/CD301b Protein, CF
This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?
The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.
Product Documents for Recombinant Mouse MGL2/CD301b Protein, CF
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