Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

Name: Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 993-MC
Price: 318 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 993-MC

Citations (10)

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Key Product Details

Source:	NS0
Accession #:	NP_038619
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse MAdCAM-1 protein

Mouse MAdCAM-1 (Gln22 - Thr365) Accession # NP_038619	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

No results obtained: Gln22 predicted

Predicted Molecular Mass

63 kDa (monomer)

SDS-PAGE

85-95 kDa, reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of HuT 78 human cutaneous T cell lymphoma cells. When 5 x 10⁴ cells/well are added to mouse MAdCAM-1/Fc Chimera coated plates (10 µg/mL with 100 µL/well), approximately 80-90% will adhere after MnCl₂ stimulation for 1 hour incubation at room temperature.
Optimal dilutions should be determined by each laboratory for each application.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

993-MC

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: MAdCAM-1

Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) is an immunoglobulin (Ig) cell adhesion molecule family member. In addition to Ig domains, it contains a mucin-like domain and a membrane proximal domain with similarity to IgA. MAdCAM-1 is involved in lymphocyte homing to mucosal sites and is expressed on high endothelial venules (HEV) of both mesenteric lymph nodes and Peyer’s patches. It has also been found to be expressed on sinus-lining cells of the spleen. The integrin, alpha ₄ beta ₇, has been shown to function as the MAdCAM-1 receptor. The Ig domains of MAdCAM-1 have been found to be critical to alpha ₄ beta ₇ binding. The mucin domain has been shown to have activity in L-Selectin binding. MAdCAM-1 expression has been demonstrated to be up-regulated by TNF-alpha and IL-1 beta. MAdCAM-1 appears to play a role in inflammatory bowel disease (IBD) as its expression is highly up-regulated in IBD and most likely serves to recruit alpha ₄ beta ₇-expressing lymphocytes to the region. In vivo studies involving nonobese diabetic (NOD) mice have also suggested that MAdCAM-1/ alpha ₄ beta ₇ interaction plays a role in diabetes development in this model. Mouse MAdCAM-1 is a 405 amino acid (aa) residue protein with a 21 aa signal sequence, a 344 aa extracellular domain, a 20 aa transmembrane domain and a 20 aa cytoplasmic domain.

Item	Value
References	Briskin, M.J. et al. (1993) Nature 363:461. Yang, X.D. et al. (1997) Diabetes 46:1542. Sampaio, S.O. et al. (1995) J. Immunol. 155:2477. Kraal, G. et al. (1995) Am. J. Pathol. 147:763. Berg, E.L. et al. (1993) Nature 366:695. Takeuchi, M. and V.R. Baichwal (1995) Proc. Natl. Acad. Sci. USA 92:3561.
Long Name	Mucosal Addressin Cell Adhesion Molecule 1
Entrez Gene IDs	8174 (Human); 17123 (Mouse)
Alternate Names	MACAM1, MAdCAM-1, MAdCAM1, hMAdCAM-1, mucosal addressin cell adhesion molecule 1, mucosal addressin cell adhesion molecule-1, mucosal vascular addressin cell adhesion molecule 1

Citations for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF (10)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

K Spinler et al. (2020-01-01), A stem cell reporter based platform to identify and target drug resistant stem cells in myeloid leukemia Nat Commun, 11(1):5998.
PMID: 33243988
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

T Savinko et al. (2018-01-01), Filamin A Is Required for Optimal T Cell Integrin-Mediated Force Transmission, Flow Adhesion, and T Cell Trafficking J. Immunol., 0(0).
PMID: 29581355
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

(2016-01-01), Differential effects of ?4?7 and GPR15 on homing of effector and regulatory T cells from patients with UC to the inflamed gut in vivo Gut, 65(10):1642-64.
PMID: 26209553
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kavanagh D et al. (2015-01-01), Pretreatment of Mesenchymal Stem Cells Manipulates Their Vasculoprotective Potential While Not Altering Their Homing Within the Injured Gut. Stem Cells, 33(9):2785-97.
PMID: 26124062
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Wang X et al. (2014-01-01), Integrin-mediated interactions between B cells and follicular dendritic cells influence germinal center B cell fitness. J Immunol, 192(10):4601-9.
PMID: 24740506
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Halpert G et al. (2014-01-01), Multifunctional activity of a small tellurium redox immunomodulator compound, AS101, on dextran sodium sulfate-induced murine colitis. J Biol Chem, 289(24):17215-27.
PMID: 24764299
Species: Mouse
Sample Types: Whole Cells
Applications: Adhesion Assay

Heidegger S et al. (2013-01-01), TLR activation excludes circulating naive CD8+ T cells from gut-associated lymphoid organs in mice. J Immunol, 190(10):5313-20.
PMID: 23589622
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Alcaide P et al. (2012-01-01), Difference in Th1 and Th17 lymphocyte adhesion to endothelium. J. Immunol., 188(3):1421-30.
PMID: 22219321
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

Arthos J et al. (2008-01-01), HIV-1 envelope protein binds to and signals through integrin alpha4beta7, the gut mucosal homing receptor for peripheral T cells. Nat. Immunol., 9(3):301-9.
PMID: 18264102
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Park EJ et al. (2007-01-01), Aberrant activation of integrin alpha4beta7 suppresses lymphocyte migration to the gut. J. Clin. Invest., 117(9):2526-38.
PMID: 17786243

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Recombinant Mouse MAdCAM-1 Fc Chimera Protein, CF

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