Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

Name: Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF
Brand: rnd
SKU: 1008-LY/CF

Catalog # 1008-LY/CF

Citations (2)

Datasheet / COA / SDS

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Key Product Details

Source:	Sf 21 (baculovirus)
Accession #:	P09225 (LT alpha ) & P41155 (LT beta )
Structure / Form:	Noncovalently-linked heterotrimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

Product Specifications

Source	Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Lymphotoxin protein Leu34-Leu202 (LT alpha ) & Gly136-Gly306 (LT beta )
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Leu34 (LT alpha ) & Gly136 (LT beta )
Predicted Molecular Mass	18.5 kDa (LT alpha ), 18.4 kDa (LT beta )
SDS-PAGE	18-22 kDa, reducing conditions
Activity	Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221. The ED₅₀ for this effect is 10-40 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1008-LY/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Lymphotoxin

Lymphotoxin-alpha (LT alpha ), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT beta ) are members of the TNF family. LT alpha cDNA encodes a 202 amino acid secreted soluble protein with a 33 amino acid signal sequence. LT beta cDNA encodes a 306 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 29 - 48), and an extracellular domain (residues 49 - 306). Secreted LT alpha assembles as a soluble homotrimer, LT alpha 3. In addition, secreted LT alpha also complexes with the membrane associated LT beta to generate two types of heterotrimers, LT alpha 1/ beta 2 and LT alpha 2/ beta 1 (1). The soluble LT alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT alpha 1/ beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT alpha 2/ beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naïve CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).

Item	Value
References	Browning et al. (1993) Cell 72:847. Ettinger et al. (1996) Proc. Natl. Acad. Sci. USA 93:13102. Cuff et al. (1998) J. Immunol. 161:6853. Browning et al. (1997) J. Immunol. 159:3288. Gramaglia et al. (1999) J. Immunol. 162:1333.
Entrez Gene IDs	4049 (Human); 16992 (Mouse)
Alternate Names	LT, Lymphotoxin, Lymphotoxin alpha, TNFSF1, TNLG1E, tnfb

Citations (2)

Showing 1- 2 of 2 citations Showing All

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF include:

Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Kayamuro H et al. (2009), TNF superfamily member, TL1A, is a potential mucosal vaccine adjuvant. Biochem. Biophys. Res. Commun., 384(3):296-300.
PMID: 19406102
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

von Zur Muhlen C et al. (2008), A contrast agent recognizing activated platelets reveals murine cerebral malaria pathology undetectable by conventional MRI. J. Clin. Invest., 118(3):1198-207.
PMID: 18274670

Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

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Product Specifications

Formulation, Preparation and Storage

Carrier Free

Carrier Free

Reconstitution Calculator

Background: Lymphotoxin

Citations (2)

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