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Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

Catalog # 1008-LY/CF | R&D Systems, Inc. a Bio-Techne Brand
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1008-LY-010/CF

Key Product Details

Source

Sf 21 (baculovirus)

Structure / Form

Noncovalently-linked heterotrimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse Lymphotoxin protein
Leu34-Leu202 (LT alpha) & Gly136-Gly306 (LT beta)

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Leu34 (LT alpha) & Gly136 (LT beta)

Predicted Molecular Mass

18.5 kDa (LT alpha), 18.4 kDa (LT beta)

SDS-PAGE

18-22 kDa, reducing conditions

Activity

Measured in a cytotoxicity assay using L‑929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. Matthews, N. and M.L. Neale (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 221.
The ED50 for this effect is 10-40 ng/mL.

Formulation, Preparation and Storage

1008-LY/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin-alpha (LT alpha), also known as tumor necrosis factor (TNF) beta, and lymphotoxin-beta (LT beta) are members of the TNF family. LT alpha cDNA encodes a 202 amino acid secreted soluble protein with a 33 amino acid signal sequence. LT beta cDNA encodes a 306 amino acid type II membrane protein with an N-terminal cytoplasmic domain (residues 1 - 18), a transmembrane region (residues 29 - 48), and an extracellular domain (residues 49 - 306). Secreted LT alpha assembles as a soluble homotrimer, LT alpha3. In addition, secreted LT alpha also complexes with the membrane associated LT beta to generate two types of heterotrimers, LT alpha1/ beta2 and LT alpha2/ beta1 (1). The soluble LT alpha3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT alpha1/ beta2, binds only to the lymphotoxin beta receptor (LT betaR). LT alpha2/ beta1 is capable of binding LT betaR, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (2, 3). Transgenic LT alpha knock-out mice exhibited a loss in lymph node development, a change in splenic architecture, and impaired germinal center formation (4). LT is expressed by activated naïve CD4 cells, unpolarized IL-2-secreting effectors, and Th1 effectors. A loss of LT expression and lack of TNF-alpha or TNF-beta secretion is associated with prior exposure to IL-4 and a Th2 phenotype (5).

References

  1. Browning et al. (1993) Cell 72:847.
  2. Ettinger et al. (1996) Proc. Natl. Acad. Sci. USA 93:13102.
  3. Cuff et al. (1998) J. Immunol. 161:6853.
  4. Browning et al. (1997) J. Immunol. 159:3288.
  5. Gramaglia et al. (1999) J. Immunol. 162:1333.

Alternate Names

LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E

Entrez Gene IDs

4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)

Gene Symbol

LTA

Additional Lymphotoxin Products

Product Documents for Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Lymphotoxin alpha2/beta1 Protein, CF

For research use only

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