Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

R&D Systems, part of Bio-Techne | Catalog # 9968-LY

R&D Systems, part of Bio-Techne
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Carrier Free
9968-LY-025/CF
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With Carrier
9968-LY-025
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Key Product Details

  • R&D Systems NS0-derived Recombinant Mouse Lymphotoxin alpha1/beta2 Protein (9968-LY)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

NS0

Accession #

P09225 (rmLT alpha); P41155 (rmLT beta)

Structure / Form

GS-linked heterotrimer

Conjugate

Unconjugated

Applications

Bioactivity

View all Lymphotoxin Proteins and Enzymes »

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Lymphotoxin protein
Mouse LT alpha
(Lys59-Leu202)
Accession # P09225		 GGGGS
 Mouse LT beta
(Leu153-Gly306) Accession # P41155
GGGGS
 Mouse LT beta
(Leu153-Gly306) Accession # P41155

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys59

Predicted Molecular Mass

50 kDa

SDS-PAGE

42-63 kDa, reducing conditions

Activity

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.3-2.1 ng/mL.

Scientific Data Images for Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

Recombinant Mouse Lymphotoxin alpha1/beta2 Protein Bioactivity

Recombinant Mouse Lymphotoxin alpha1/beta2 Protein Bioactivity

Recombinant Mouse Lymphotoxin alpha1/ beta2 (Catalog # 9968-LY) induces NIH‑3T3 mouse embryonic fibroblast cell proliferation. The ED50 for this effect is 0.3-2.1 ng/mL.
Recombinant Mouse Lymphotoxin alpha1/beta2 Protein SDS-PAGE

Recombinant Mouse Lymphotoxin alpha1/beta2 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse Lymphotoxin alpha1/beta2 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 42-63 kDa.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 9968-LY
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.
Carrier Free: 9968-LY/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: Lymphotoxin

Lymphotoxin alpha (LT alpha) and Lymphotoxin beta (LT beta) are pro-inflammatory TNF superfamily ligands that play important roles in immune system development (1, 2). Mouse LT alpha cDNA encodes a 202 amino acid (aa) secreted soluble protein with a 33 aa signal sequence. The mature mouse LT alpha shares 75% aa sequence identity with human LT alpha (3, 4). Mouse LT beta cDNA encodes a 306 aa type II membrane protein with a 27 aa N-terminal cytoplasmic domain, a 21 aa transmembrane region, and a 258 aa extracellular domain. It shares 73% aa sequence identity with human LT beta within common regions of their extracellular domains (5). Secreted LT alpha assembles as a soluble homotrimer, LT alpha 3. In addition, secreted LT alpha also complexes with the membrane associated LT beta to generate two types of heterotrimers, LT alpha 1/beta 2 and LT alpha 2/beta 1 (5). The soluble LT alpha 3 binds both TNF RI (p55) and TNF RII (p75). In contrast, the predominant membrane-bound heterotrimer, LT alpha 1/beta 2, binds only to the lymphotoxin beta receptor (LT beta R). LT alpha 2/beta 1 is capable of binding LT beta R, TNF RI (p55), and TNF RII (p75). LT plays a role in normal lymphoid organogenesis (6, 7). The LT alpha 1/beta 2 heterotrimer binds and activates the LT beta R/TNFRSF3 which is expressed on macrophages, dendritic cells, hepatocytes, intestinal epithelial cells (IEC), follicular dendritic cells (FDC), and high endothelial venules (HEV) (2, 8, 9). LT beta R also serves as a receptor for LIGHT/TNFSF14 (10). LT alpha 1/beta 2 promotes the development of FDC networks and HEV in lymphoid tissue, the class switching of immature B cells for IgA production, and the production of homeostatic IL-22 by ILCs (11-14). It can be shed by ADAM17 or MMP-8 mediated cleavage, and the released heterotrimer circulates in the serum and is elevated in synovial fluid of rheumatoid arthritis patients (15).

References

  1. Lu, T.T. and J.L. Browning (2014) Front. Immunol. 5:47.
  2. Upadhyay, V. and Y.-X. Fu (2014) Cytokine Growth Factor Rev. 25:227.
  3. Gray, P.W. et al. (1984) Nature 312:721.
  4. Nedwin, G.E. et al. (1985) J. Cell Biochem. 29:171.
  5. Browning, J.L. et al. (1993) Cell 72:847.
  6. Ettinger R. et al. (1996) Proc. Natl. Acad. Sci. USA 93:13102.
  7. Cuff, C.A. et al. (1998) J. Immunol. 161:6853.
  8. Sudhamsu, J. et al. (2013) Proc. Natl. Acad. Sci. USA 110:19896.
  9. Crowe, P.D. et al. (1994) Science 264:707.
  10. Eldredge, J. et al. (2006) Biochemistry 45:10117.
  11. Kruglov, A.A. et al. (2013) Science 342:1243.
  12. Futterer, A. et al. (1998) Immunity 9:59.
  13. Koni, P.A. et al. (1997) Immunity 6:491.
  14. Ota, N. et al. (2011) Nat. Immunol. 12:941.
  15. Young, J. et al. (2010) Cytokine 51:78.

Alternate Names

LT, Lymphotoxin alpha, tnfb, TNFSF1, TNLG1E

Entrez Gene IDs

4049 (Human); 16992 (Mouse); 102138497 (Cynomolgus Monkey)

Gene Symbol

LTA

UniProt

P09225 (rmLT alpha); P41155 (rmLT beta)

Additional Lymphotoxin Products

Product Documents for Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

Product Datasheets

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Download SDS 9968-LY (with carrier)
Download SDS 9968-LY/CF (carrier free)

Product Specific Notices for Recombinant Mouse Lymphotoxin alpha1/beta2 Protein

For research use only

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