Recombinant Mouse LRP-4 His-tag Protein, CF

Name: Recombinant Mouse LRP-4 His-tag Protein, CF
Brand: rnd
SKU: 10229-LR

Catalog # 10229-LR

Datasheet / COA / SDS

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Key Product Details

Source:	HEK293
Accession #:	Q8VI56.3
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse LRP-4 His-tag Protein, CF

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse LRP-4 protein

Mouse LRP-4 (Ser21-Ser1725) Accession # Q8VI56.3	HPGGGSGGGSGGGS	HHHHHH
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ser21

Predicted Molecular Mass

192 kDa

SDS-PAGE

198-235 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse USAG-1 (Catalog # 9008-SD) is present at 2 μg/mL, the concentration of Recombinat Mouse LRP‑4 that produces 50% of the optimal binding response is 80-800 ng/mL.

Recombinant Mouse LRP-4 His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse LRP-4 His-tag Protein Binding Activity When Recombinant Mouse USAG1 (Catalog # 9008-SD) is used at 2 µg/mL (100 µL/well), it binds to Recombinant Mouse LRP-4 (Catalog # 10229-LR) with an ED₅₀ of 80-800 ng/mL.
	Recombinant Mouse LRP-4 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse LRP-4 His-tag was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 180-235 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10229-LR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LRP-4

LDL receptor-related protein 4 (LRP-4), also known as Megf7, is an approximately 220 kDa type I membrane protein in the LDL receptor superfamily. LRP-4 plays an important role in development of the neuromuscular junction (NMJ), bones, teeth, mammary placodes, and hair follicles (1, 2). Mature mouse LRP-4 consists of a 1705 amino acid (aa) extracellular domain (ECD), a 21 aa transmembrane segment, and a 159 aa cytoplasmic domain. The ECD contains 3 EGF-like domains, 8 LDLR-A domains, and 20 LDLR-B repeats that fold into 4 beta-propeller domains (3, 4). Within the ECD, mouse LRP-4 shares 97% and 99% aa sequence identity with human and rat LRP-4, respectively. The ECD of LRP-4 can be shed by ADAM10 mediated cleavage (5). LRP-4 is expressed on myotubes (6, 7), neurons (8, 9), osteocytes and osteoblasts (10, 11), developing tooth epithelium (12), and male and female germ cells (13). It is localized to NMJ on developing myotubes, and it associates in cis with the muscle-specific kinase MuSK (6, 7). Its binding to neuron-derived Agrin promotes additional LRP-4 association with MuSK, MuSK activation, clustering of AChR, and postsynaptic development of the NMJ (6, 7, and 14). LRP-4 also enhances presynaptic differentiation of the NMJ, although this does not require Agrin (14). Neuronal LRP-4 additionally binds ApoE and Fspondin (8, 9). LRP-4 regulates both BMP and Wnt signaling through binding to Wise, Sclerostin, and Dkk1 (10, 12). These interactions are important for the control of bone growth and mineralization, tooth morphogenesis, and the development of mammary placodes, hair follicles, and whiskers (10, 12, and 15).

Item	Value
References	Zong, Y. and R. Jin (2012) Cell. Mol. Life Sci. Nov 22 Epub. Ohazama, A. et al. (2010) Am. J. Med. Genet. A 152A:2974. Nakayama, M. et al. (1998) Genomics 51:27. Tomita, Y. et al. (1998) J. Biochem. 124:784. Dietrich, M.F. et al. (2010) PLoS ONE 5:e9960. Kim, N. et al. (2008) Cell 135:334. Zhang, B. et al. (2008) Neuron 60:285. Lu, Y. et al. (2007) Brain Res. 1177:19. Zisman, S. et al. (2007) J. Cell Biol. 178:1237. Leupin, O. et al. (2011) J. Biol. Chem. 286:19489. Choi, H.Y. et al. (2009) PLoS ONE 4:e7930. Ohazama, A. et al. (2008) PLoS ONE 3:e4092. Yamaguchi, Y.L. et al. (2006) Gene Expr. Patterns 6:607. Yumoto, N. et al. (2012) Nature 489:438. Ahn, Y. et al. (2013) Development 140:583.
Long Name	LDL Receptor-related Protein 4
Entrez Gene IDs	4038 (Human); 228357 (Mouse); 83469 (Rat)
Alternate Names	CLSS, KIAA0816, LRP-4, LRP10, LRP4, MEGF7LRP10, Multiple epidermal growth factor-like domains 7, low density lipoprotein receptor-related protein 4, low-density lipoprotein receptor-related protein 4

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