Recombinant Mouse LAMP-2/CD107b Fc Chimera Protein, CF

Name: Recombinant Mouse LAMP-2/CD107b Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10552-LM

Catalog # 10552-LM

Product Datasheet / COA / SDS

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Key Product Details

Source:	NS0
Accession #:	P17047.2
Structure / Form:	Dilsulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse LAMP-2/CD107b Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse LAMP-2/CD107b protein

Mouse LAMP-2/CD107b (Leu26-Asn379) Accession # P17047.2	IEGRMDP	Mouse IgG_2a (Glu98-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu26

Predicted Molecular Mass

66 kDa

SDS-PAGE

109-122 kDa, under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When Recombinant Mouse LAMP‑2/CD107b Fc Chimera is immobilized at 1 μg/mL (100 μL/well), it binds to Recombinant Human Galectin-3 (Catalog # 1154-GA) with an ED₅₀ of 0.1-10 μg/mL.

Recombinant Mouse LAMP-2/CD107b Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse LAMP-2/CD107b Fc Chimera Protein Binding Activity When Recombinant Mouse LAMP‑2/CD107b Fc Chimera (Catalog # 10552-LM) is immobilized at 1 μg/mL (100 μL/well), it binds to Recombinant Human Galectin-3 (1154-GA) with an ED₅₀ of 0.1-10 μg/mL
	Recombinant Mouse LAMP-2/CD107b Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse LAMP-2/CD107b Fc Chimera (Catalog # 10552-LM) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 109-122 kDa and 210-240 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10552-LM

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: LAMP-2/CD107b

Lysosomal associated membrane protein 2 (LAMP-2), also known as CD107b and LGP110, is an approximately 110 kDa transmembrane glycoprotein that is a major component of lysosomal membranes (1). Mature mouse LAMP-2 consists of a 354 amino acid (aa) intralumenal domain, a 25 aa transmembrane segment, and a 11 aa cytoplasmic tail (2). Its lumenal domain is organized into two heavily N-glycosylated regions separated by a Ser/Pro-rich linker that carries a minor amount of O‑linked glycosylation (2, 3). Alternate splicing generates two additional mouse LAMP-2 isoforms (LAMP-2B and LAMP-2C) with a substituted juxtamembrane lumenal region, transmembrane segment, and cytoplasmic tail (4). Within the lumenal domain, mouse LAMP-2 shares approximately 64% and 81% aa sequence identity with human and rat LAMP-2, repectively. LAMP-2 itself is subject to lysosomal degradation following cleavage of its lumenal domain (5). It mediates the lysosomal uptake of the chaperone HSC73 in complex with cargo proteins and is required for the lysosomal destruction of autophagic vacuoles (6, 7). In cytotoxic T cells and mast cells, LAMP-2 is expressed in the membranes of intracellular granules that contain effector molecules such as perforin, granzymes, eicosanoids, and histamine (8-10). Up‑regulated LAMP-2 at the plasma membrane serves as an indicator of cell activation of CD8+ T cells, mast cells, monocytes, and platelets (9-12). LAMP-2 is a native ligand for lectins Galectin-1 and Galectin-3 (13‑15).

Item	Value
References	Eskelinen, E.-L. et al. (2003) Trends Cell Biol. 13:137. Fukuda, M. et al. (1988) J. Biol. Chem. 263:18920. Carlsson, S.R. et al. (1988) J. Biol. Chem. 263:18911. Lichter-Konecki, U. et al. (1999) Differentiation 65:43. Cuervo, A.M. and J.F. Dice (2000) Traffic 1:570. Cuervo, A.M. and J.F. Dice (1996) Science 273:501. Tanaka, Y. et al. (1990) Nature 406:902. Peters, P.J. et al. (1991) J. Exp. Med. 173:1099. Betts, M.R. et al. (2003) J. Immunol. Meth. 281:65. Grutzkau, A. et al. (2004) Cytometry 61:62. Kannan, K. et al. (1996) Cell. Immunol. 171:10. Silverstein, R.L. and M. Febbraio (1992) Blood 80:1470. Skrincosky, D.M. et al. (1993) Cancer Res. 53:2667. Inohara, H. and Raz, A. (1994) Biochem. Biophys. Res. Commun. 201:1366. Ohannesian D.W. et al. (1994) Cancer Res. 54:5992.
Long Name	Lysosome-associated Membrane Glycoprotein 2
Entrez Gene IDs	3920 (Human); 16784 (Mouse)
Alternate Names	CD107 antigen-like family member B, CD107b, CD107b antigen, LAMP-2, LAMP-2A, LAMP2, LAMP2A, LAMPB, LGP-96, LGP110, Lysosomal Associated Membrane Protein 2 , Lysosome-associated membrane protein 2, lysosomal-associated membrane protein 2, lysosome-associated membrane glycoprotein 2

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