Product Specifications for Recombinant Mouse Kirrel2/NEPH3 Protein, CF
Mouse myeloma cell line, NS0-derived mouse Kirrel2/NEPH3 protein Ser19-Asp501, with a C-terminal 10-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
66 kDa, reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of MS‑1 mouse pancreatic islet endothelial cells. When 5 x 104 cells/well are added to rmKirrel2 coated plates (30 µg/mL, 100 µL/well), approximately 30-60% will adhere after 90 minutes at 37° C.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin superfamily (1, 2). Kirrel2 is one of several proteins expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1 - 9). Mouse Kirrel2 cDNA encodes a 700 amino acid (aa) protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589 - 598) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% aa identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35 - 37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501 - 533), while another encodes only portions of Ig domains 1 - 4 (5). Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (6). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet beta cell function (7). In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (8). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (9).
Sun C. et al. (2003) Genomics 82:130.
Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.
Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.
Sellin, L. et al. (2003) FASEB J. 17:115.
SwissProt Accession # Q7TSU7.
Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.
Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.
Serizawa, S. et al. (2006) Cell 127:1057.
Minaki, Y. et al. (2005) Neurosci. Res. 52:250.
Kin of Irregular Chiasm-like 2, Splice Variant A
Entrez Gene IDs
84063 (Human); 243911 (Mouse)
DKFZp564A1164, FILTRINkin of IRRE-like protein 2, Filtrin, Kin of irregular chiasm-like protein 2, Kirrel2, MGC15718, NEPH3, NEPH3Nephrin-like protein 3, NLG1, NLG1kin of irregular chiasm-like 2, kin of IRRE like 2 (Drosophila), nephrin-like 3, nephrin-like gene 1
Citations for Recombinant Mouse Kirrel2/NEPH3 Protein, CF
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Product Documents for Recombinant Mouse Kirrel2/NEPH3 Protein, CF
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