Key Product Details
Lys20-Lys354, with a C-terminal 6-His tag
N-terminal Sequence Analysis
Predicted Molecular Mass
When Recombinant Mouse IL1RAPL2 His-tag (Catalog # 11164-MR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (Catalog # 9995-PR) binds with an ED50 of 80.0-800 ng/mL.
Scientific Data Images for Recombinant Mouse IL1RAPL2 His-tag Protein, CF
Recombinant Mouse IL1RAPL2 His-tag Protein Binding Activity.When Recombinant Mouse IL1RAPL2 His-tag Protein (Catalog # 11164-MR) is immobilized at 1 µg/mL (100 µL/well), Recombinant Human PTPRD Fc Chimera (9995-PR) binds with an ED50 of 80.0-800 ng /mL.
Recombinant Mouse IL1RAPL2 His-tag Protein SDS-PAGE.2 μg/lane of Recombinant Mouse IL1RAPL2 His-tag Protein (Catalog # 11164-MR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 45-62 kDa.
Formulation, Preparation and Storage
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.|
|Reconstitution||Reconstitute at 500 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
The Interleukin 1 receptor family (IL-1R) comprises at least eleven members including IL-1 RI (IL-1R1), IL-1 RII (IL-1 R2), IL-1 RAcP (IL-1 R3), ST2 (T1/IL‑1 R4), IL‑18 R alpha (IL-1 Rrp/IL-1 R5), IL-1 Rrp2 (IL-1 RL2/IL-1 R6), IL-18 R beta (AcPL/IL-1 R7), IL1RAPL1 (TIGIRR-2/IL-1 R8), and IL1RAPL2 (TIGIRR-1/IL-1 R9) (1). All family members possess three immunoglobulin (Ig)-like domains in their extracellular region. Most members also have an intracellular TIR (Toll-like receptor/IL-1 receptor signaling) domain that is also conserved in the Toll-like receptor family. Related proteins, SIGIRR (single Ig domain-containing IL-1 R-related molecule) and IL-18BP, differ from the other members by having only one Ig domain (1). IL-1 receptor accessory protein-like 2 (IL1RAPL2) is alternately known as IL-1 R9 and three immunoglobulin domain containing IL-1 receptor-related molecule 1 (TIGIRR-1) and is expressed in the brain (2). Human IL1RAPL2 is a type I transmembrane glycoprotein with a 16 aa signal peptide, a 338 aa extracellular region containing three Ig-like domains, an 18 aa transmembrane domain and a 311 aa cytoplasmic tail (3). By comparison to other IL-1 receptor family proteins, IL1RAPL2 has a C-terminal cytoplasmic extension beyond the TIR domain that is found in IL-1RAPL1 and SIGIRR but not other family members (3). Human and mouse IL1RAPL2 share approximately 95% aa sequence identity. Human IL1RAPL2 is most homologous (63%) to IL-1RAPL1, a receptor protein that is highly expressed in hippocampus and is involved in X-linked mental retardation (4, 5). Genes for both have been localized to human chromosome Xq22. A ligand for IL1RAPL2 has not been identified (1).
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Andre, R. et al. (2005) J. Neurochem. 95:324.
- Born, T.L. et al. (2000) J. Biol. Chem. 275:29946.
- Jin, H. et al. (2000) Eur. J. Hum. Genet. 8:87.
- Carrie, A. et al. (1999) Nat. Genet. 23:25.
Product Specific Notices for Recombinant Mouse IL1RAPL2 His-tag Protein, CF
For research use only