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Recombinant Mouse IL-9 (HEK293 expressed) Protein, CF

Bio-Techne includes R&D Systems | Catalog # 11391-ML

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11391-ML-010
11391-ML-050
11391-ML-250

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IL-9 protein
Gln19-Pro144

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Gln19 (Blocked), reveal Ala20 after deblocking

Predicted Molecular Mass

14 kDa

SDS-PAGE

35-39 kDa, under reducing conditions.

Activity

Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. Avanzi, G. et al. (1988) Br. J. Haematol. 69:359.
The ED50 for this effect is 1.50-15.0 ng/mL.

Scientific Data Images for Recombinant Mouse IL-9 (HEK293 expressed) Protein, CF

Recombinant Mouse IL‑9 (HEK293 expressed) Protein Bioactivity.

Recombinant Mouse IL‑9 (HEK293 expressed) Protein (Catalog # 11391-ML) induces MO7e human megakaryocytic leukemic cell proliferation. The ED50 for this effect is 1.50‑15.0 ng/mL.

Recombinant Mouse IL‑9 (HEK293 expressed) Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse IL‑9 (HEK293 expressed) Protein (Catalog # 11391-ML) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 35-39 kDa, under reducing conditions.

Formulation, Preparation and Storage

11391-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution Reconstitute the 10 μg size at 100 μg/mL in PBS. Reconstitute all other sizes at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-9

Interleukin-9 (IL-9), also known as P40 and MEA (mast cell growth-enhancing activity), is a 30-40 kDa glycosylated member of a cytokine family that includes Interleukins-2, -4, -7, -15, and -21. These proteins utilize heteromeric receptors containing the Common gamma chain ( gammac) in addition to ligand-specific subunits. IL-9 interacts selectively with IL-9 R which then associates with gammac to form the functional receptor complex. IL-9 contributes to allergic inflammation, autoimmunity-induced inflammation, parasite clearance from the GI tract, and Treg-mediated immune suppression (1, 2). It enhances the expansion and recruitment of mast cells and eosinophils as well as the production of IgE and Th2 cytokines (3‑6). It is required for anaphylactic responses to ingested allergens but not to systemic allergens (7). IL-9 plays multiple roles in the development and function of subsets within the CD4+ T cell lineage (8). It is expressed by activated Th9, Th17, Treg, and Th2 cells (3, 9‑12). IL-9 acts as an autocrine growth and activation factor for Th17, Treg, and mast cells (3, 11, 13). It also can inhibit immune responses by enhancing the suppressive properties of Treg and by recruiting immune-suppressive mast cells to sites of inflammation (11, 12). Mature mouse IL-9 shares 57% and 74% amino acid sequence identity with human and rat IL-9, respectively (14).

References

  1. Noelle, R.J. and E.C. Nowak (2010) Nat. Rev. Immunol. 10:683.
  2. Goswami, R. and M.H. Kaplan (2011) J. Immunol. 186:3283.
  3. Nowak, E.C. et al. (2009) J. Exp. Med. 206:1653.
  4. Townsend, M.J. et al. (2000) Immunity 13:573.
  5. Leech, M.D. and R.K. Grencis (2006) J. Immunol. 176:2505.
  6. Fawaz, L.M. et al. (2007) J. Allergy Clin. Immunol. 120:1208.
  7. Osterfeld, H. et al. (2010) J. Allergy Clin. Immunol. 125:469.
  8. Jabeen, R. and M.H. Kaplan (2012) Curr. Opin. Immunol. 24:303.
  9. Tan, C. et al. (2010) J. Immunol. 185:6795.
  10. Jager, A. et al. (2009) J. Immunol. 183:7169.
  11. Elyaman, W. et al. (2009) Proc. Natl. Acad. Sci. USA 106:12885.
  12. Lu, L.-F. et al. (2006) Nature 442:997.
  13. Stassen, M. et al. (2000) J. Immunol. 164:5549.
  14. Van Snick, J. et al. (1989) J. Exp. Med. 169:363.

Long Name

Interleukin 9

Alternate Names

IL9

Entrez Gene IDs

3578 (Human); 16198 (Mouse)

Gene Symbol

IL9

UniProt

Additional IL-9 Products

Product Documents for Recombinant Mouse IL-9 (HEK293 expressed) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-9 (HEK293 expressed) Protein, CF

For research use only

Reconstitution Calculator

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