Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Name: Recombinant Mouse IL-6/IL-6R alpha Protein Chimera
Brand: R&D Systems
SKU: 9038-SR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9038-SR

Citations (3)

Product Datasheet / COA / SDS

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Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	P22272 (IL-6 R alpha ) & P08505 (IL-6)
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Product Specifications for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Source

Mouse myeloma cell line, NS0-derived mouse IL-6/IL-6R alpha Complex protein

Mouse IL-6R alpha (Leu20-Glu357) Accession # P22272	GGGSGGGSGGGS	Mouse IL-6 (Phe25-Thr211) Accession # P08505
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Leu20 (major), Leu28 (minor)

Predicted Molecular Mass

60 kDa

SDS-PAGE

70-87 kDa, reducing conditions

Activity

Measured by its ability to inhibit M1 mouse myeloid leukemia cell proliferation. The ED₅₀ for this effect is 2‑12 ng/mL.

Scientific Data Examples for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

	Recombinant Mouse IL-6/IL-6R alpha Protein Chimera Bioactivity Recombinant Mouse IL-6/IL-6R alpha complex (Catalog # 9038-SR) inhibits M1 mouse myeloid leukemia cell proliferation. The ED₅₀ for this effect is 2-12 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

9038-SR

Carrier

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

9038-SR/CF

Carrier Free

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-6/IL-6R alpha Complex

Interleukin-6 (IL-6) is a pleiotropic, alpha -helical, 22-28 kDa phosphorylated and variably glycosylated cytokine that plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6, along with TNF-alpha and IL-1, drives the acute inflammatory response and the transition from acute inflammation to either acquired immunity or chronic inflammatory disease (1-5). When dysregulated, it contributes to chronic inflammation in obesity, insulin resistance, inflammatory bowel disease, arthritis, sepsis, and atherosclerosis (1-3). IL-6 can also function as an anti-inflammatory molecule, as in skeletal muscle where it is secreted in response to exercise (2). In addition, it enhances hematopoietic stem cell proliferation and the differentiation of Th17 cells, memory B cells, and plasma cells (1, 6). IL-6 induces signaling through a cell surface heterodimeric receptor complex composed of a ligand binding subunit (IL-6R alpha) and a signal transducing subunit (gp130). IL-6 binds to IL-6 R alpha, triggering IL-6 R alpha association with gp130 and gp130 dimerization (7). gp130 is also a component of the receptors for CLC, CNTF, CT-1, IL-11, IL-27, LIF, and OSM (8). Soluble forms of IL-6 R alpha are generated by both alternative splicing and proteolytic cleavage (3). In a mechanism known as trans-signaling, complexes of soluble IL-6 and IL-6 R alpha elicit responses from gp130-expressing cells that lack cell surface IL-6 R alpha (3). Trans-signaling enables a wider range of cell types to respond to IL-6, as the expression of gp130 is ubiquitous, while that of IL-6 R alpha is predominantly restricted to hepatocytes, monocytes, and resting lymphocytes (2, 3). Soluble splice forms of gp130 block trans-signaling from IL-6/IL-6 R alpha but not from other cytokines that use gp130 as a co-receptor (3, 9). Mature mouse IL-6 shares 39% and 85% amino acid (aa) sequence identity with human and rat IL-6, respectively (10-12). Within the extracellular domain, mouse IL-6 R alpha shares 51% and 89% aa sequence identity with human and rat IL-6 R alpha, respectively (13).

Item	Value
References	Mansell, A. and B.J. Jenkins (2013) Cytokine Growth Factor Rev. 24:249. Schuett, H. et al. (2009) Thromb. Haemost. 102:215. Hunter, C.A. and S.A. Jones (2015) Nat. Immunol. 16:448. Erta, M. et al. (2012) Int. J. Biol. Sci. 8:1254. Garbers, C. et al. (2012) Cytokine Growth Factor Rev. 23:85. Cerutti, A. et al. (1998) J. Immunol. 160:2145. Murakami, M. et al. (1993) Science 260:1808. Muller-Newen, G. (2003) Sci. STKE 2003:PE40. Mitsuyama, K. et al. (2006) Clin. Exp. Immunol. 143:125. Chiu, C.P. et al. (1988) Proc. Natl. Acad. Sci. USA 85:7099. Simpson, R.J. et al. (1988) Eur. J. Biochem. 176:187. Van Snick, J. et al. (1988) Eur. J. Immunol. 18:193. Sugita, T. et al. (1990) J. Exp. Med. 171:2001.
Long Name	Interleukin 6/Interleukin 6 Receptor alpha Complex
Entrez Gene IDs	3570 (Human); 16194 (Mouse); 24499 (Rat)
Alternate Names	IL-6/IL-6R alpha Complex

Citations for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera include:

Species: Human
Sample Types: Whole Cells
Applications: Cell Culture

MB Lutz et al. (2022), Guidelines for mouse and human DC generation European Journal of Immunology, 0(0).
PMID: 36303448
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

P Innamarato et al. (2020), Reactive Myelopoiesis Triggered by Lymphodepleting Chemotherapy Limits the Efficacy of Adoptive T Cell Therapy Mol. Ther., 0(0).
PMID: 32615068
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

E Xu et al. (2017), Temporal and tissue-specific requirements for T-lymphocyte IL-6 signalling in obesity-associated inflammation and insulin resistance Nat Commun, 8(0):14803.
PMID: 28466852

Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

R&D Systems, Inc. a Bio-Techne Brand

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Product Specifications for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Scientific Data Examples for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Formulation, Preparation and Storage

Carrier Free

9038-SR

Carrier

9038-SR/CF

Carrier Free

Reconstitution Calculator

Background: IL-6/IL-6R alpha Complex

Citations for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera (3)

Product Documents for Recombinant Mouse IL-6/IL-6R alpha Protein Chimera

Product Datasheets

COA

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SDS

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