Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF

Name: Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 553-MR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 553-MR

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	P21183
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

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Product Specifications for Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse IL-5 R alpha/CD125 protein

Mouse IL-5 R alpha (Asp18-His339) Accession # P21183	IEGRMD	Human IgG₁ (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Asp18

Predicted Molecular Mass

63.2 kDa (monomer)

SDS-PAGE

75-85 kDa, 55 kDa and 35 kDa under reducing conditions

Activity

Measured by its binding ability in a functional ELISA. When 0.5 μg/mL of Recombinant Mouse IL‑5 R alpha /CD125 Fc Chimera is immobilized onto a goat anti-human IgG Fc antibody coated plate, the concentration of biotinylated recombinant mouse IL-5 that produces 50% of the optimal binding response is found to be approximately 4‑16 ng/mL

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

553-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1-3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha, respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8-10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

Item	Value
References	Martinez-Moczygemba, M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653. Rothenberg, M.E. and S.P. Hogan (2005) Annu. Rev. Immunol. 24:147. Elsas, X.P. and M.I.G. Elsas (2007) Curr. Med. Chem. 14:1925. Takaki, S. et al. (1990) EMBO J. 9:4367. Cameron, L. et al. (2000) J. Immunol. 164:1538. Tavernier, J. et al. (1991) Cell 66:1175. Zaks-Zilberman, M. et al. (2008) J. Biol. Chem. 283:13398. Tavernier, J. et al. (2000) Blood 95:1600. Clutterbuck, E.J. et al. (1989) Blood 73:1504. Lopez, A.F. et al. (1988) J. Exp. Med. 167:219. Denburg, J.A. et al. (1991) Blood 77:1462. Hasbold, J. et al. (2004) Nat. Immunol. 5:55. Gregory, B. et al. (2003) J. Immunol. 170:5359. Liu, L.Y. et al. (2002) J. Immunol. 169:6459.
Long Name	Interleukin 5 Receptor alpha
Entrez Gene IDs	3568 (Human); 16192 (Mouse)
Alternate Names	CD125, CD125 antigen, CDw125CD125, HSIL5R3, IL-5 R alpha, IL-5 receptor subunit alpha, IL-5R subunit alpha, IL-5R-alpha, IL-5Ra, IL5R, IL5R alpha, IL5RA, MGC26560, interleukin 5 receptor type 3, interleukin 5 receptor, alpha, interleukin-5 receptor alpha chain, interleukin-5 receptor alpha subunit, interleukin-5 receptor subunit alpha

Citations for Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: cell culture
Applications: Bioassay

BT Stocks et al. (2019), Regulation of Diabetogenic Immunity by IL15-Activated Regulatory CD8 T Cells in Type 1 Diabetes J. Immunol., 0(0).
PMID: 31127035

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Recombinant Mouse IL-5 R alpha Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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