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Recombinant Mouse IL-36Ra/IL-1F5 Protein

Catalog # 2714-ML | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
2714-ML-025/CF

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With Carrier
2714-ML-025

Key Product Details

Source

E. coli

Accession #

Conjugate

Unconjugated

Applications

Binding Activity, Bioactivity

Product Specifications

Source

E. coli-derived mouse IL-36Ra/IL-1F5 protein
Val2-Asp156

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val2

Predicted Molecular Mass

17 kDa

Activity

Measured by its ability to inhibit IL-36 alpha, IL-36 beta or IL-36 gamma-induced IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells.
The ED50 for this effect is 0.8-4 μg/mL in the presence of 15 ng/mL of Recombinant Mouse IL‑36 beta/IL‑1F8 (aa 31-183) (Catalog # 7060-ML).

Measured by its binding ability in a functional ELISA.
Immobilized Recombinant Mouse IL-36Ra/IL-1F5 at 1 µg/mL (100 µL/well) can bind Recombinant Human IL-1 Rrp2/IL-1 R6 Fc Chimera(Catalog # 872-RP) with a linear range of 0.15-5 µg/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2714-ML
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT with BSA as a carrier protein.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2714-ML/CF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS and DTT.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36Ra/IL-1F5

Mouse interleukin-36 receptor antagonist (IL-36Ra; previously IL-1F5; also named FIL-1 delta [delta], IL‑1HY1, IL-1H3, and IL-1L1) is a member of the IL‑1 family of proteins (1-6). IL‑1 family members include IL-1 beta, IL-1 alpha, IL-1ra, IL‑18 and IL‑1F5 through F10 (6, 7). All family members show a 12 beta-strand, beta-trefoil configuration, and are believed to have arisen from a common ancestral gene that underwent multiple duplications (8). The mouse IL‑36Ra/IL‑1F5 gene maps to a region on mouse chromosome 2 that contains all other IL-1 family members (except IL‑18), supporting an evolutionary relationship with the IL-1 family (1, 9). It is particularly close to the gene for IL-1ra and is likely a relatively recent duplication of that gene. IL‑36Ra/IL‑1F5 is synthesized as a 156 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (2, 5, 8). Nevertheless, it appears to be secreted as a 17 kDa monomer. In humans, there is an alternate start site that potentially gives rise to an alternate splice form (5). This translated product has a premature stop codon, resulting in a truncated 16 aa peptide. Mouse to human, full length IL‑36Ra/IL‑1F5 has 90% aa identity. Within the family, IL-36Ra/IL-1F5 is 48%, 30%, 35%, 35%, 35%, 37% and 43% aa identical to IL‑1ra, IL‑1 beta, IL‑36 alpha/IL‑1F6, IL‑37/IL‑1F7, IL-36 beta/IL-1F8, IL‑36 gamma/IL‑1F9 and IL‑1F10, respectively. Cells reported to express IL-36Ra/IL-1F5 include monocytes, B cells, dendritic cells/Langerhans cells, keratinocytes, and gastric fundus Parietal and Chief cells (1, 8). The receptor for IL-36Ra/IL-1F5 has not been positively identified. Indirect evidence suggests it is IL‑1 Rrp2 and/or IL-1 RAcP (9). In either case, activity association with receptor binding is unclear. It was initially reported to be an antagonist of  IL-36 gamma activity (4, 7). This would be consistent with its hypothesized relationship to IL‑1ra.

References

  1. Smith, D. E. et al. (2000) J. Biol. Chem. 275:1169.
  2. Kumar, S. et al. (2000) J. Biol. Chem. 275:10308.
  3. Mulero, J.J. et al. (1999) Biochem. Biophys. Res. Commun. 263:702.
  4. Nicklin, M.J.H. et al. (2002) Genomics. 79:718.
  5. Barton J. L. et al. (2000) Eur. J. Immunol. 30:3299.
  6. Dinarello, C. et al. (2010) Nat. Immunol. 11:973.
  7. Dunn, E. et al. (2001) Trends Immunol. 22:533.
  8. Debets, R. et al. (2001) J. Immunol. 167:1440.
  9. Towne, J.E. et al. (2004) J. Biol. Chem. 279:13677.

Long Name

Interleukin 36 Receptor Antagonist/Interleukin 1 Family 5

Alternate Names

FIL1 delta, IL-1HY1, IL-1L1, IL-1RP3, IL1F5, IL36Ra, IL36RN

Entrez Gene IDs

26525 (Human); 54450 (Mouse)

Gene Symbol

IL36RN

UniProt

Product Documents for Recombinant Mouse IL-36Ra/IL-1F5 Protein

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-36Ra/IL-1F5 Protein

For research use only

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