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Recombinant Mouse IL-31RA Fc Chimera Protein, CF

Catalog # 1253-IL | R&D Systems, Inc. a Bio-Techne Brand
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1253-IL-050

Key Product Details

Source

Sf 21 (stably transfected)

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Spodoptera frugiperda, Sf 21 (stably transfected)-derived mouse IL-31 RA protein
Mouse IL-31RA
(Val19-Ser503)
Accession # AAM27959.1
DIEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val19

Predicted Molecular Mass

82 kDa

SDS-PAGE

90-103 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Mouse IL‑31 RA Fc Chimera is immobilized at 5 µg/mL (100 µL/well), Recombinant Mouse IL‑31 (Catalog # 3028-ML) binds with an ED50 of 0.9-5.4 μg/mL.

Scientific Data Images for Recombinant Mouse IL-31RA Fc Chimera Protein, CF

Recombinant Mouse IL-31RA Fc Chimera Protein SDS-PAGE

Recombinant Mouse IL-31RA Fc Chimera Protein SDS-PAGE

1 μg/lane of Recombinant Mouse IL‑31 RA was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by silver staining, showing bands at 90-103 kDa and 180-210 kDa, respectively.

Formulation, Preparation and Storage

1253-IL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
  • 12 months from date of receipt, ≤ -20 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: IL-31RA

The interleukin-31 receptor A subunit (IL-31 RA), also known as gp130-Like Monocyte Receptor (GLM-R or GPL), is a ~100 kDa type I transmembrane glycoprotein that is classified as being a type I cytokine receptor (1, 2). A heterodimeric complex of IL-31 RA and the oncostatin M receptor (OSM R) functions as the signaling receptor for IL-31 (3). Both subunits are inducibly expressed throughout the myelomonocytic lineage and are up-regulated by interferon-gamma and bacterial lipopolysaccharides (1-3). IL-31 RA is also expressed on keratinocytes, dorsal root ganglia neurons, and variably on lung epithelial cells (3-6). The 716 amino acid (aa) mouse IL-31 RA contains an 18 aa signal sequence, a 481 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 196 aa cytoplasmic domain. The ECD of mouse IL-31 RA shares 60% and 84% aa identity with human and rat IL-31 RA ECD, respectively. Mouse IL-31 receptors do not respond to human IL-31 (7). The ECD contains five fibronectin type III domains; the first two contain four conserved cysteine residues and a WSXWS motif common to type I cytokine receptors (2).  In both humans and transgenic mice, IL-31 from skin-homing Th2 cells may contribute to the pruritis (itching) associated with nonatopic dermatitis, especially in infected skin (3, 8, 9).

References

  1. Ghilardi, N. et al. (2002) J. Biol. Chem. 277:16831.
  2. Diveu, C. et al. (2003) J. Biol. Chem. 278:49850.
  3. Dillon, S. R. et al. (2004) Nat. Immunol. 5:752.
  4. Chattopadhyay, S. et al. (2007) J. Biol. Chem. 282:3014.
  5. Perrigoue, J. G. et al. (2007) J. Exp. Med. 204:481.
  6. Bando, T. et al. (2006) Neuroscience 142:1263.
  7. Broxmeyer, H. E. et al. (2007) Exp. Hematol. 35:78.
  8. Bilsborough, J. et al. (2006) J. Allergy Clin. Immunol. 117:418.
  9. Sonkoly, E. et al. (2006) J. Allergy Clin. Immunol. 117:411.

Long Name

Interleukin 31 Receptor A

Alternate Names

CRL3, GLM-R, IL-31 RA, IL31RA

Entrez Gene IDs

133396 (Human); 218624 (Mouse)

Gene Symbol

IL31RA

UniProt

Additional IL-31RA Products

Product Documents for Recombinant Mouse IL-31RA Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-31RA Fc Chimera Protein, CF

For research use only

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