Recombinant Mouse IL-21R Fc Chimera Protein, CF

Name: Recombinant Mouse IL-21R Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 596-MR

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 596-MR

Citations (30)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	NS0
Accession #:	Q9JHX3
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse IL-21R Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse IL-21R Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse IL-21 R protein

Mouse IL-21 R Subunit (Cys20 - Pro236) Accession # Q9JHX3	IEGRMD	Human IgG₁ (Pro100 - Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Cys20

Predicted Molecular Mass

51.5 kDa (monomer)

SDS-PAGE

70-80 kDa, reducing conditions

Activity

Measured by its ability to inhibit IL-21-dependent enhancement of IFN-gamma secretion in NK-92 human natural killer lymphoma cells. The ED₅₀ for this effect is 75-500 ng/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

596-MR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: IL-21R

IL-21 R (interleukin-21 receptor) is a type I transmembrane glycoprotein within the class I cytokine receptor family, type 4 subfamily (1 ‑ 5). Complex formation between IL-21 R and the common gamma chain ( gamma _c), also used for IL-2, IL-4, IL-7, IL-9, IL-13 and IL-15 receptors, is required for signaling (6, 7). Mouse IL-21 R cDNA encodes 521 amino acid (aa) including a 19 aa signal peptide, a 218 aa extracellular domain (ECD) with 4 conserved cysteine residues, a fibronectin type III domain, and a WSXWS motif, a 21 aa transmembrane domain and a 271 aa cytoplasmic domain with a Box 1 motif, a kinase domain, and several sites for tyrosine phosphorylation (4, 5). One such site, pY510, mediates STAT binding (1, 2). The mouse IL‑21 R ECD shares 69%, 91%, 65%, 63% and 58% aa identity with human, rat, equine, canine and bovine IL-21 R, respectively. One potential 447 aa isoform, with an alternate start site at aa 83, lacks the four conserved ECD cysteines. IL-21 R is expressed mainly on B cells (highest on mature, activated, follicular and germinal center B cells), NK cells, and activated T cells, but is also found on dendritic cells, alternatively activated macrophages, intestinal lamina propria fibroblasts and epithelial cells, and keratinocytes (1, 3 ‑ 5). Both IL‑21 and IL‑4 are necessary for efficient B cell IgG1 production and normal germinal center architecture (8). B cell IL‑21 R engagement induces Blimp-1 (which mediates plasma cell differentiation), and is important for memory responses (1, 9, 10). IL-21 R engagement on mouse NK cells enhances their cytotoxic activity and IFN-gamma production (4, 11). IL‑21 R engagement on CD8⁺ T cells aids control of viral infection and tumor growth; IL-21 R is also necessary for sufficient numbers of regulatory T cells to combat chronic inflammation (1, 12, 13). IL‑21 R expression is often up‑regulated in allergic skin inflammation, systemic lupus erythematosus and diffuse large B cell lymphoma (DLBCL) (1, 2, 14, 15).

Item	Value
References	Leonard, W.J. et al. (2008) J. Leukoc. Biol. 84:348. Konforte, D. et al. (2009) J. Immunol. 182:1791. Monteleone, G. et al., 2009, Cytokine Growth Factor Rev. 20:185. Parrish-Novak, et al. (2000) Nature 408:57. Ozaki, K. et al. (2000) Proc. Natl. Acad. Sci. USA 97:11439. Asao, H. et al. (2001) J. Immunol. 167:1. Habib, T. et al. (2002) Biochemistry 41:8725. Ozaki, K. et al. (2002) Science 298:1630. Rankin, A.L. et al. (2011) J. Immunol. 186:667. King, I.L. et al. (2010) J. Immunol. 185:6138. Kasaian, M.T. et al. (2002) Immunity 16:559. Frohlich, A. et al. (2009 Science 324:1576. Tortola, L. et al. (2010) Blood 116:5200. Jin, H. et al. (2009) J. Clin. Invest. 119:47. Sarosiek, K.A. et al. (2010) Blood 115:570.
Long Name	Interleukin 21 Receptor
Entrez Gene IDs	50615 (Human); 60504 (Mouse); 102135042 (Cynomolgus Monkey); 705759 (Rhesus Macaque)
Alternate Names	CD360, CD360 antigen, IL-21 R, IL-21 receptor, IL-21R, IL21R, MGC10967, NILR, Novel interleukin receptor, interleukin 21 receptor, interleukin-21 receptor

Citations for Recombinant Mouse IL-21R Fc Chimera Protein, CF (30)

Showing 1 - 10 of 30 citations Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IL-21R Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

D Cortés-Sel et al. (2021), Maternal schistosomiasis impairs offspring Interleukin-4 production and B cell expansion PloS Pathogens, 17(2):e1009260.
PMID: 33524040
Species: Mouse
Sample Types: Whole Cells
Applications: ICC

LE Latham et al. (2020), ICOS signaling promotes a secondary humoral response after re-challenge with Plasmodiumï¿½chabaudi chabaudi AS PLoS Pathog., 16(4):e1008527.
PMID: 32348365
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

JS Park et al. (2019), Interleukin-21-mediated suppression of the Pax3-Id3 pathway exacerbates the development of Sjï¿½gren's syndrome via follicular helper T cells Cytokine, 125(0):154834.
PMID: 31491724
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

MD Powell et al. (2019), IL-12 signaling drives the differentiation and function of a TH1-derived TFH1-like cell population Sci Rep, 9(1):13991.
PMID: 31570752
Species: Mouse
Sample Types: Whole Cells
Applications: Cell Culture

L Wang et al. (2019), Control of Germinal Center Localization and Lineage Stability of Follicular Regulatory T Cells by the Blimp1 Transcription Factor Cell Rep, 29(7):1848-1861.e6.
PMID: 31722202
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

KM Valentine et al. (2018), CD8 Follicular T Cells Promote B Cell Antibody Class Switch in Autoimmune Disease J. Immunol., 0(0).
PMID: 29743314
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

D Cortes-Sel et al. (2018), IL-4 Promotes stromal cell expansion and Is critical for development Of A Type-2, but not a type 1 immune response Eur. J. Immunol., 0(0).
PMID: 30575951
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

CT Johndrow et al. (2018), Suppression of Th1 Priming by TLR2 Agonists during Cutaneous Immunization Is Mediated by Recruited CCR2+ Monocytes J. Immunol., 0(0).
PMID: 30455402
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Q Cheng et al. (2018), Neddylation contributes to CD4+ T cell-mediated protective immunity against blood-stage Plasmodium infection PLoS Pathog., 14(11):e1007440.
PMID: 30462731
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

JY Choi et al. (2017), Disruption of Pathogenic Cellular Networks by IL-21 Blockade Leads to Disease Amelioration in Murine Lupus J. Immunol, 0(0).
PMID: 28219887
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Jang E et al. (2016), Splenic Long-Lived Plasma Cells Promote the Development of Follicular Helper T Cells during Autoimmune Responses. J Immunol, 196(3):1026-35.
PMID: 26729802
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Wikenheiser D et al. (2016), The Costimulatory Molecule ICOS Regulates Host Th1 and Follicular Th Cell Differentiation in Response to Plasmodium chabaudi chabaudi AS Infection. J Immunol, 196(2):778-91.
PMID: 26667167
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2016), Antigen-presenting cell-derived IL-6 restricts the expression of GATA3 and IL-4 by follicular helper T cells J Leukoc Biol, 0(0).
PMID: 27474166
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Weber J et al. (2015), ICOS maintains the T follicular helper cell phenotype by down-regulating Kruppel-like factor 2. J Exp Med, 212(2):217-33.
PMID: 25646266
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Kim Y et al. (2015), Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells. PLoS ONE, 10(3):e0120294.
PMID: 25768299
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Preite S et al. (2015), Somatic mutations and affinity maturation are impaired by excessive numbers of T follicular helper cells and restored by Treg cells or memory T cells. Eur J Immunol, 45(11):3010-21.
PMID: 26332258
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Teichmann L et al. (2015), Local triggering of the ICOS coreceptor by CD11c(+) myeloid cells drives organ inflammation in lupus. Immunity, 42(3):552-65.
PMID: 25786178
Species: Mouse
Sample Types: Whole Cells
Applications: Binding Assay

Perez-Mazliah D et al. (2015), Disruption of IL-21 signaling affects T cell-B cell interactions and abrogates protective humoral immunity to malaria. PLoS Pathog, 11(3):e1004715.
PMID: 25763578
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Fairfax K et al. (2015), IL-4-secreting secondary T follicular helper (Tfh) cells arise from memory T cells, not persisting Tfh cells, through a B cell-dependent mechanism. J Immunol, 194(7):2999-3010.
PMID: 25712216
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Giles J et al. (2015), B Cell-Specific MHC Class II Deletion Reveals Multiple Nonredundant Roles for B Cell Antigen Presentation in Murine Lupus. J Immunol, 195(6):2571-9.
PMID: 26268653
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Vogelzang et al. (2014), IL-21 contributes to fatal inflammatory disease in the absence of Foxp3+ T regulatory cells. J Immunol, 192(4):1404-14.
PMID: 24446516
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

Beverley P et al. (2014), A novel murine cytomegalovirus vaccine vector protects against Mycobacterium tuberculosis. J Immunol, 193(5):2306-16.
PMID: 25070842
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Ikeda S et al. (2014), Excess IL-1 signaling enhances the development of Th17 cells by downregulating TGF-beta-induced Foxp3 expression. J Immunol, 192(4):1449-58.
PMID: 24431229
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Conter et al. (2014), CD73 expression is dynamically regulated in the germinal center and bone marrow plasma cells are diminished in its absence. PLoS ONE, 9(3):e92009.
PMID: 24664100
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Debuisson D et al. (2013), Myor/ABF-1 mRNA [corrected] Expression Marks Follicular Helper T Cells but Is Dispensable for Tfh Cell Differentiation and Function In Vivo. PLoS ONE, 8(12):e84415.
PMID: 24386375
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

(2012), T-follicular helper cells survive as long-term memory cells. Eur. J. Immunol., 42(8):1981-8.
PMID: 22730020
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Boettler et al. (2012), OX40 facilitates control of a persistent virus infection. PLoS Pathog, 8(9):e1002913.
PMID: 22969431
Species: Mouse
Sample Types: In Vivo
Applications: In Vivo

McGuire HM et al. (2011), A subset of interleukin-21+ chemokine receptor CCR9+ T helper cells target accessory organs of the digestive system in autoimmunity. Immunity, 34(4):602-15.
PMID: 21511186
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Kashiwakuma D et al. (2010), B and T lymphocyte attenuator suppresses IL-21 production from follicular Th cells and subsequent humoral immune responses. J. Immunol., 185(5):2730-6.
PMID: 20660710
Species: Mouse
Sample Types: Whole Cells
Applications: Flow Cytometry

Poholek AC et al. (2010), In vivo regulation of Bcl6 and T follicular helper cell development. J. Immunol., 185(1):313-26.
PMID: 20519643

There are no citations that match your criteria.

Showing 1 - 10 of 30 citations Showing All

Product Documents for Recombinant Mouse IL-21R Fc Chimera Protein, CF

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Recombinant Mouse IL-21R Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

Key Product Details

Customers also Viewed

Customers also Purchased