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Recombinant Mouse IL-17F Protein

Catalog # 2057-IL | R&D Systems, Inc. a Bio-Techne Brand
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Carrier Free
2057-IL-025/CF

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With Carrier
2057-IL-025

Key Product Details

Source

E. coli

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

E. coli-derived mouse IL-17F protein
Arg21-Ala153, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

15 kDa (monomer)

SDS-PAGE

16 kDa, reducing conditions

Activity

Measured by its ability to induce IL-6 secretion by NIH‑3T3 mouse embryonic fibroblast cells. Yao, Z. et al. (1995) Immunity 3:811.
The ED50 for this effect is 20-120 ng/mL.

Formulation, Preparation and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 2057-IL
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 2057-IL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution Reconstitute at 100 μg/mL in sterile 4 mM HCl.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-17F

The Interleukin 17 (IL-17) family proteins, comprised of six members (IL-17, IL-17B through IL-17F), are secreted, structurally related proteins that share a conserved cysteine-knot fold near the C-terminus, but have considerable sequence divergence at the N-terminus. With the exception of IL-17B, which exists as a non-covalently linked dimer, all IL-17 family members are disulfide-linked dimers. IL-17 family proteins are pro-inflammatory cytokines that induce local cytokine production and are involved in the regulation of immune functions (1, 2).

Mouse IL-17F cDNA encodes a 153 amino acid (aa) protein with a putative 20 aa signal peptide. Among IL-17 family members, IL-17F is most closely related to IL-17 sharing approximately 46% aa sequence identity. Mouse and human IL-17F share 55% sequence identity. IL-17F is expressed in activated CD4+ T cells and activated monocytes. Two receptors (IL-17 R, and IL-17B R), which are activated by IL-17 family members have been identified. In addition, at least three additional type I transmembrane receptors with homology to IL-17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have also been reported (1, 2, 5). The functions for IL‑17 RC, D, and E are not known. Purified IL-17 R and IL-17B R do not bind IL-17F with high-affinity in vitro. However, binding of IL-17F is detected in cells transfected with IL-17 R, raising the possibility that a co-receptor may be required for IL-17F signaling through IL-17 R (4). The biological activities mediated by IL-17F are similar to those of IL-17. IL-17F stimulates production of IL-6, IL-8, G-CSF, and regulates cartilage matrix turnover by increasing matrix release and inhibiting new matrix synthesis (4). IL-17F also inhibits angiogenesis and induces production of IL-2, TGF-beta, and monocyte chemoattractant protein-1 in endothelial cells (3).

References

  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Starnes, T. et al. (2001) J. Immunol. 167:4137.
  4. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
  5. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.

Long Name

Interleukin 17F

Alternate Names

IL17F

Entrez Gene IDs

112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

Gene Symbol

IL17F

UniProt

Additional IL-17F Products

Product Documents for Recombinant Mouse IL-17F Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IL-17F Protein

For research use only

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