Recombinant Mouse IGFBP-6 Protein, CF

Name: Recombinant Mouse IGFBP-6 Protein, CF
Brand: R&D Systems
SKU: 776-B6

Catalog # 776-B6 | R&D Systems, Inc. a Bio-Techne Brand

Citations (1)

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Key Product Details

Source:	NS0
Accession #:	P47880
Applications:	Bioactivity

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Technical Data
Citations (1) / Reviews

Product Specifications for Recombinant Mouse IGFBP-6 Protein, CF

Source	Mouse myeloma cell line, NS0-derived mouse IGFBP-6 protein Gly24-Gly238 & Gly29-Gly238
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin level	<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Gly24 & Gly29
Predicted Molecular Mass	23 kDa
SDS-PAGE	23 kDa
Activity	Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED₅₀ for this effect is 0.4‑2 µg/mL in the presence of 30 ng/mL rmIGF-II.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

776-B6

Formulation	Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA.
Reconstitution	Reconstitute at 200 μg/mL in sterile PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

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Background: IGFBP-6

The superfamily of insulin-like growth factor (IGF) binding proteins include the six high-affinity IGF binding proteins (IGFBP) and at least four additional low-affinity binding proteins referred to as IGFBP related proteins (IGFBP-rP). All IGFBP superfamily members are cysteine-rich proteins with conserved cysteine residues, which are clustered in the amino- and carboxy-terminal thirds of the molecule. IGFBPs modulate the biological activities of IGF proteins. Some IGFBPs may also have intrinsic bioactivity that is independent of their ability to bind IGF proteins. Post-translational modifications of IGFBPs, including glycosylation, phosphorylation and proteolysis, have been shown to modify the affinities of the binding proteins to IGF.

Mouse IGFBP-6 cDNA encodes a 238 amino acid (aa) residue precursor protein with a putative 25 aa residue signal peptide that is processed to generate the 213 aa residue mature protein that is O-glycosylated. Mouse and human IGFBP-6 share 73% amino acid similarity. Mouse and rat IGFBP-6 share 94% amino acid similarity and the mouse IGFBP-6 has a 9 amino acid insertion compared to the rat homolog. IGFBP-6 is expressed in ovarian, testicular, muscle, heart and lung tissues in the adult mouse. IGFBP-6 was not detected in total RNA from a whole mouse embryo.

Item	Value
References	Schuller, A.G.P. et al. (1994) Mol. Cell. Endoc. 104:57. Jones, J.I. and D.R. Clemmons (1995) Endocrine Rev. 16:3. Kelley, K.M. et al. (1996) Int. J. Biochem. Cell Biol. 28:619.
Long Name	Insulin-like Growth Factor Binding Protein 6
Entrez Gene IDs	3489 (Human); 16012 (Mouse)
Alternate Names	IBP-6, IBP6, IGF binding protein 6, IGF-binding protein 6, IGFBP-6, IGFBP6, insulin-like growth factor binding protein 6, insulin-like growth factor-binding protein 6

Citations for Recombinant Mouse IGFBP-6 Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IGFBP-6 Protein, CF include:

Sample Types: N/A
Applications: Enzyme Assay

Larsen PH et al. (2006-02-22), Myelin formation during development of the CNS is delayed in matrix metalloproteinase-9 and -12 null mice. J. Neurosci., 2006-026(8):2207-14.
PMID: 16495447

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