Chinese Hamster Ovary cell line, CHO-derived mouse IFNAA protein Cys24-Glu190
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
16-21 kDa, under reducing conditions
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is <15 pg/mL.
Recombinant Mouse IFNAA Protein, CF Scientific Data Examples
Recombinant Mouse IFNAA Protein Bioactivity
Recombinant Mouse IFNAA (Catalog # 10150-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is <15 pg/mL.
Recombinant Mouse IFNAA Protein SDS-PAGE
2 μg/lane of Recombinant Mouse IFNAA (Catalog # 10150-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 16-21 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Reconstitute at 100 μg/mL in PBS.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Interferon-alpha A (IFNAA), also referred to as interferon-alpha 15 (IFNA15), is one of 14 subtypes within the IFN-alpha family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFNAR2 and IFNAR1 subunits (2). The mature extracellular domain (ECD) of mouse IFNAA is 167 aa and shares 93% and 92% aa sequence identity with mouse and rat, respectively. Most of the members of the murine IFN-alpha family are N-glycosylated, however IFNAA is one of 4 members that lacks a N-glycosylation site (3). IFNAA is one of the many interferon alpha subtypes belonging to the Type I leukocyte IFN family. The Type I IFN family has antiviral, anti-proliferative and natural killer cell activities.
Interferon alpha A
Entrez Gene IDs
Gm12597, IFNA15, IFNAA
Pestka, S. (2007) J Biol Chem. 282:20047.
Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
Pesch, V. et al. (2004). Journal of Virology, 78:8219.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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