Product Specifications for Recombinant Mouse IFN-beta (Mammalian) Protein, CF
Gene was obtained from mouse DNA expressed in mammalian cells. Accession # K00020
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
<1.0 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass
Interferon was titrated with the use of the cytopathic effect inhibition assay as listed
Mouse (L929/EMCV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~2.5 U/ml. Lot Activity was derived from multiple determinations in the above assay. The units are determined by use of a Mu-IFN-beta laboratory standard calibrated to the international reference standard for mouse interferon beta provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1- 2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
20 mM Sodium Phosphate, pH 6.4; 300 mM NaCl; 5% Glycerol
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.
Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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