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Recombinant Mouse IFN-beta (Mammalian) Protein, CF

Catalog # 12410-1 | R&D Systems, Inc. a Bio-Techne Brand
A New rm IFN-beta is Available! It has lower endotoxin specifications, is made in-house, and is priced competitively!
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12410-1

Key Product Details

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source


Gene was obtained from mouse DNA expressed in mammalian cells.
Accession # K00020

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Predicted Molecular Mass

19.6 kDa

Activity

Interferon was titrated with the use of the cytopathic effect inhibition assay as listed

Mouse (L929/EMCV) – performed as described [Rubinstein, et al. (1981) J. Virol. 37(2):755]. The EC50 for interferon in this assay is ~2.5 U/ml. Lot Activity was derived from multiple determinations in the above assay. The units are determined by use of a Mu-IFN-beta laboratory standard calibrated to the international reference standard for mouse interferon beta provided by the National Institutes of Health [Meager, et al. (2001) J. Immunol Methods. 257 (1- 2):17-33]. Please note that IFN assays vary between labs and assay systems [Meager, et al. (2001). J. Immunol. Meth. 257:17. Meager and Das (2005) J. Immunol. Meth. 306:1].

Formulation, Preparation and Storage

12410-1
Formulation 20 mM Sodium Phosphate, pH 6.4; 300 mM NaCl; 5% Glycerol
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IFN-beta

Interferon-beta (IFN-beta) is a cytokine that is produced by fibroblasts and pathogen-exposed dendritic cells, macrophages, and endothelial cells. It signals through the heterodimeric IFN-alpha/beta Receptor. IFN-beta-deficient mice show increased susceptibility to experimental autoimmune encephalomyelitis (EAE), a disease model of human multiple sclerosis (MS). Furthermore, IFN-beta has been shown to suppress the Th17 cell response in both MS and EAE and has commonly been used as a treatment for MS.

Long Name

Interferon beta

Alternate Names

IFB, IFF, IFNB, IFNB1, IFNbeta

Entrez Gene IDs

3456 (Human); 15977 (Mouse); 24481 (Rat)

Gene Symbol

IFNB1

UniProt

Additional IFN-beta Products

Product Documents for Recombinant Mouse IFN-beta (Mammalian) Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IFN-beta (Mammalian) Protein, CF

For research use only

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