Source	Chinese Hamster Ovary cell line, CHO-derived mouse IFN-alpha 7/IFNA7 protein Cys24-Glu190
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
Predicted Molecular Mass	19 kDa
SDS-PAGE	18-24 kDa, under reducing conditions
Activity	Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀ for this effect is <1.5 ng/mL.

Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF Scientific Data Examples

	Recombinant Mouse IFN-alpha 7/IFNA7 Protein Bioactivity Recombinant Mouse IFN-alpha 7/IFNA7 (Catalog # 10208-IJ) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is <1.5 ng/mL.
	Recombinant Mouse IFN-alpha 7/IFNA7 Protein SDS-PAGE 2 μg/lane of Recombinant Mouse IFN-alpha J1/IFNA7 (Catalog # 10208-IJ) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-24 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10208-IJ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha 7/IFNA7

Interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, classified based on their binding specificity to cell surface receptors (1). There are more than a dozen closely related IFN alpha subtypes found in both the human and mouse genome, each sharing about 80% amino acid (aa) sequence homology (2, 3). Mature mouse IFNA7 consists of 166 aa and shares 59% identity with human IFNA7. The type I IFNs bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 (alpha -subunit) and IFNAR2 (beta -subunit) (4, 5). Individual IFN alpha subtypes are known to display unique efficacies to viral protection, with IFNA7 showing slightly lower antiviral potency against Mengo virus but maintaining similar anti-proliferative potency in B16 melanoma cells (5, 6).

Item	Value
References	Pestka S, et al. (1987) Annu Rev Biochem. 56:727. Matsumiya, T. et al. (2007) J. Immunol. 179:4542. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36:139. Fung, K.Y. et al. (2013) Science 339:1088. van Pesch, V. et al. (2004) J. Virol. 78:8219. James CM et al. (2007) Vaccine. 25(10):1856.
Long Name	Interferon alpha 7
Entrez Gene IDs	3444 (Human); 15970 (Mouse)
Alternate Names	IFN-alpha 7, IFN-alpha J1, IFNA7, IFNalpha J1, interferon alpha-7, interferon alpha-J1, interferon, alpha 7

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Recombinant Mouse IFN-alpha 7/IFNA7 Protein, CF

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