Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 2/IFNA2 protein Cys24-Glu190
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
18-22 kDa, under reducing conditions
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 20-200 pg/mL.
Recombinant Mouse IFN-alpha 2/IFNA2 Protein, CF Scientific Data Examples
Recombinant Mouse IFN-alpha 2/IFNA2 Protein Bioactivity
Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 20-200 pg/mL.
Recombinant Mouse IFN-alpha 2/IFNA2 Protein SDS-PAGE
2 μg/lane of Recombinant Mouse IFN-alpha 2/IFNA2 (Catalog # 10149-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
3 months, -20 to -70 °C under sterile conditions after opening.
Background: IFN-alpha 2/IFNA2
Interferon-alpha 2 (IFN alpha -2) is one of 14 subtypes within the IFN-alpha class of Type I Interferons (1). The members of the IFN-alpha class, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1, 3, 4). IFN-alpha class members signal through a common cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (3). As the first highly active IFN to be cloned and produced, IFN alpha -2 has become the prototypic IFN for academic and pharmaceutical research (2). The mature extracellular domain (ECD) of mouse IFN alpha -2 shares 60% and 83% aa sequence identity with human and rat, respectively. Murine IFN-alpha 2 can eliminate cardiac viral load and protect cardiomyocytes from injury in animals infected with coxsackievirus B3 (CVB3) (5). IFN alpha -2 derived mutants with reduced IFNR2 binding inhibited HIV replication and mutants with more IFNAR1 binding potentiated antiviral activity (6).
Pestka, S. (2007) J Biol Chem. 282:20047.
Paul, F. et al. (2015) Gene. 567(2):132.
Oritani, K. et al. (2001). Cytokine & Growth Factor Reviews, 12:337.
Pesch, V. et al. (2004). Journal of Virology, 78:8219.
Wang, Y.X. et al. (2007) Am J Physiol Heart Circ Physiol. 293:H69.
Schlaepfer, E. et al. (2019) Am Soc for Microbiology 4:e00637.
Which Brands are Currently Available on bio-techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments and Simple Plex Assays are available to quote. ProteinSimple branded instrument consumables and ACD branded products will be available on bio-techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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