Product Specifications for Recombinant Mouse IFN-alpha 12 Protein, CF
Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 12 protein Cys24-Glu189
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
18-25 kDa, under reducing conditions
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED50 for this effect is 10-100 pg/mL.
Scientific Data Examples for Recombinant Mouse IFN-alpha 12 Protein, CF
Recombinant Mouse IFN-alpha 12 Protein Bioactivity
Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 10-100 pg/mL.
Recombinant Mouse IFN-alpha 12 Protein SDS-PAGE
2 μg/lane of Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-25 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-alpha 12
Interferon-alpha 12 (IFN alpha -12) is a member of the type I interferon-alpha (IFN-alpha ) family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (2). The mature extracellular domain (ECD) of mouse IFN alpha -12 is 166 aa. While IFNa-12 does not exist in humans, it closely resembles other murine members of the IFN-alpha family, such as sharing 88% and 86% sequence identity with IFN alpha -15 and IFN alpha -11 respectively. Murine IFN-alpha 12 differs from other members of the IFN-alpha family by its anti-proliferative function and in the inability of its expression to be regulated by viral infection (5).
Moulton, V. R. (2017) Trends Mol Med. 23:615.
Oritani, K. et al. (2001) Cytokine & Growth Factor Reviews. 12:337.
Pesch, V. et al. (2004). Journal of Virology. 78:8219.
Hardy, M. P. et al. (2004). Genomics, 84:331.
Tsang, S. L. et al. (2007). Cytokine, 37:101.
Interferon alpha 12
Entrez Gene IDs
IFN-alpha 12, IFNA12, Interferon alpha 12
Citations for Recombinant Mouse IFN-alpha 12 Protein, CF
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Product Documents for Recombinant Mouse IFN-alpha 12 Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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