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Recombinant Mouse IFN-alpha 12 Protein, CF

Catalog # 10370-IF | R&D Systems, Inc. a Bio-Techne Brand
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10370-IF-010
10370-IF-050

Key Product Details

Source

HEK293

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 12 protein
Cys24-Glu189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Cys24

Predicted Molecular Mass

19.2 kDa

SDS-PAGE

18-25 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 10-100 pg/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 12 Protein, CF

Recombinant Mouse IFN-alpha 12 Protein Bioactivity

Recombinant Mouse IFN-alpha 12 Protein Bioactivity

Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 10-100 pg/mL.
Recombinant Mouse IFN-alpha 12 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 12 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 12 (Catalog # 10370-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-25 kDa.

Formulation, Preparation and Storage

10370-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IFN-alpha 12

Interferon-alpha 12 (IFN alpha-12) is a member of the type I interferon-alpha (IFN-alpha) family (1). The members of the IFN-alpha family, also known as alpha leukocyte interferons, encompass a group of distinct but closely related proteins which share approximately 80% amino acid (aa) sequence identity and have a similar globular structure composed of five alpha-helices (1-3). IFN-alpha family members signal through a common set of cell surface receptor complex composed of IFN-alpha R2 and IFN-alpha R1 subunits (2). The mature extracellular domain (ECD) of mouse IFN alpha-12 is 166 aa. While IFNa-12 does not exist in humans, it closely resembles other murine members of the IFN-alpha family, such as sharing 88% and 86% sequence identity with IFN alpha-15 and IFN alpha-11 respectively. Murine IFN-alpha 12 differs from other members of the IFN-alpha family by its anti-proliferative function and in the inability of its expression to be regulated by viral infection (5).

References

  1. Moulton, V. R. (2017) Trends Mol Med. 23:615.
  2. Oritani, K. et al. (2001) Cytokine & Growth Factor Reviews. 12:337.
  3. Pesch, V. et al. (2004). Journal of Virology. 78:8219.
  4. Hardy, M. P. et al. (2004). Genomics, 84:331.
  5. Tsang, S. L. et al. (2007). Cytokine, 37:101.

Long Name

Interferon alpha 12

Alternate Names

IFNA12, Interferon alpha 12

Entrez Gene IDs

242519 (Mouse)

Gene Symbol

IFNA12

UniProt

Product Documents for Recombinant Mouse IFN-alpha 12 Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse IFN-alpha 12 Protein, CF

For research use only

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