Source	Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 1 protein Cys24-Lys189
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained. Cys24 inferred from enzymatic pyroglutamate treatment revealing Asp25.
Predicted Molecular Mass	19 kDa
SDS-PAGE	18-22 kDa, under reducing conditions
Activity	Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681. The ED₅₀for this effect is 1.2-12 pg/mL.

Recombinant Mouse IFN-alpha 1 Protein, CF Scientific Data Examples

	Recombinant Mouse IFN-alpha 1 Protein Bioactivity Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED₅₀ for this effect is 1.2-12 pg/mL.
	Recombinant Mouse IFN-alpha 1 Protein SDS-PAGE 2 μg/lane of Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10148-IF

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 100 μg/mL in PBS.
Shipping	The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 3 months, -20 to -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-alpha 1

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and classified based on their binding specificity to cell surface receptors (1). The type I IFN bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha -subunit) and IFNAR2 ( beta -subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFNa subtypes and the varous IFN a share about 80% sequence homology among them (4-5). Interferon-alpha 1 (IFNA1) is a secreted, approximately 19 kDa member of the type I interferon family of molecules (6). Mature mouse IFN-alpha 1 shares 63% and 82% amino acid sequence identity with human and rat IFN-alpha 1, respectively. Low level IFN-alpha is detected under physiological conditions, and the production of IFNs is markedly enhanced during virus infection (7). Although originally discovered by its capability to fight virus replication, IFN-alpha functions as a prototypic tumor suppressor that represses the clinical tumorigenic phenotype in some malignancies (7).

Item	Value
References	Pestka, S. et al. (1987) Annu Rev Biochem. 56:727. Fung, K.Y. et al. (2013) Science 339:1088. Matsumiya, T. et al. (2007) J. Immunol. 179:4542. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36: 139. van Pesch, V. et al. (2004) J. Virol. 78: 8219. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412. Gutterman J. U. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:1198.
Long Name	Interferon alpha 1
Entrez Gene IDs	3439 (Human); 15962 (Mouse); 690894 (Rat)
Alternate Names	IFN-alpha 1, IFNA1, IFNalpha 1

Citations for Recombinant Mouse IFN-alpha 1 Protein, CF (1)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse IFN-alpha 1 Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

GM Wiedemann et al. (2021), Deconvoluting global cytokine signaling networks in natural killer cells Nature Immunology, 0(0).
PMID: 33859404

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Recombinant Mouse IFN-alpha 1 Protein, CF

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