Recombinant Mouse IFN-alpha 1 Protein, CF

Catalog # 10148-IF | R&D Systems, Inc. a Bio-Techne Brand
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Key Product Details

Accession #

P01572

Source

HEK293

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Human embryonic kidney cell, HEK293-derived mouse IFN-alpha 1 protein
Cys24-Lys189

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained. Cys24 inferred from enzymatic pyroglutamate treatment revealing Asp25.

Predicted Molecular Mass

19 kDa

SDS-PAGE

18-22 kDa, under reducing conditions

Activity

Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Vogel, S.N. et al. (1982) Infect. Immunol. 38:681.
The ED50 for this effect is 1.2-12 pg/mL.

Scientific Data Images for Recombinant Mouse IFN-alpha 1 Protein, CF

Recombinant Mouse IFN-alpha 1 Protein Bioactivity

Recombinant Mouse IFN-alpha 1 Protein Bioactivity

Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) suppresses viral activity on L-929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. The ED50 for this effect is 1.2-12 pg/mL.
Recombinant Mouse IFN-alpha 1 Protein SDS-PAGE

Recombinant Mouse IFN-alpha 1 Protein SDS-PAGE

2 μg/lane of Recombinant Mouse IFN-alpha 1 (Catalog # 10148-IF) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 18-22 kDa.

Formulation, Preparation and Storage

10148-IF
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 3 months, -20 to -70 °C under sterile conditions after opening.

Background: IFN-alpha 1

The interferons (IFN) are a family of cytokines with potent antiviral, antiproliferative and immunomodulatory properties, and classified based on their binding specificity to cell surface receptors (1). The type I IFN bind to the interferon alpha receptor (IFNAR), which consists of two subunits: IFNAR1 ( alpha-subunit) and IFNAR2 ( beta-subunit). This binding contributes to TNF-alpha induced signaling (2, 3). Both the human and mouse genome code for more than a dozen closely related IFNa subtypes and the varous IFN a share about 80% sequence homology among them (4-5). Interferon-alpha 1 (IFNA1) is a secreted, approximately 19 kDa member of the type I interferon family of molecules (6). Mature mouse IFN-alpha 1 shares 63% and 82% amino acid sequence identity with human and rat IFN-alpha 1, respectively. Low level IFN-alpha is detected under physiological conditions, and the production of IFNs is markedly enhanced during virus infection (7). Although originally discovered by its capability to fight virus replication, IFN-alpha functions as a prototypic tumor suppressor that represses the clinical tumorigenic phenotype in some malignancies (7).

References

  1. Pestka, S. et al. (1987) Annu Rev Biochem. 56:727.
  2. Fung, K.Y. et al. (2013) Science 339:1088.
  3. Matsumiya, T. et al. (2007) J. Immunol. 179:4542.
  4. Schreiber, G. and J. Piehler (2015) Trends Immunol. 36: 139.
  5. van Pesch, V. et al. (2004) J. Virol. 78: 8219.
  6. Wijesundara, D.K. et al. (2014) Front. Immunol. 5:412.
  7. Gutterman J. U. et al. (1994) Proc. Natl. Acad. Sci. U. S. A. 91:1198.

Long Name

Interferon alpha 1

Alternate Names

IFNA1, IFNalpha 1

Entrez Gene IDs

3439 (Human); 15962 (Mouse); 690894 (Rat)

Gene Symbol

IFNA1

UniProt

P01572

Product Documents for Recombinant Mouse IFN-alpha 1 Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse IFN-alpha 1 Protein, CF

For research use only

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