Product Specifications for Recombinant Mouse HPRG Protein, CF
Mouse myeloma cell line, NS0-derived mouse HPRG protein Leu19-Lys525, with a C-terminal 10-His tag
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
Measured by its ability to support the adhesion of MOLT‑4 human acute lymphoblastic leukemia cells. Lamb-Wharton, R.J. and W.T. Morgen (1993) Cell Immunol. 152:544. When 1 x 105 cells/well are added to Recombinant Mouse HPRG coated plates, adhesion is induced in a dose-dependent manner in the presence of Concanvalin A. The ED50 for this effect is 0.6-3 μg/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in MES and NaCl.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Mouse histidine-rich glycoprotein (HPRG) is a multidomain, monomeric, secreted, 67 - 75 kDa member of the cystatin superfamily of molecules (1, 2). Its name derives from the fact that 22% of its amino acids (aa) are histidine and proline. In mouse, it is synthesized as a 525 amino acid (aa) precursor that contains an 18 aa signal sequence and a 507 aa mature region (3). Five distinct domains are recognized in the mature molecule. There are two N-terminal cystatin-like modules and one His-Pro-rich region that is flanked by two Pro-rich segments (3, 4). The His-Pro-rich region contains multiple tandem repeats with a GHHPH motif, while the N- and C-termini are linked by a disulfide bond (3, 5, 6). Mouse HPRG is only 60% aa identical to human HPRG, and 79% aa identical to rat HPRG. There are multiple ligands for HPRG. These include small molecular weight molecules (metal ions; heme), hemostatic molecules (heparan sulfate; TSP; plasminogen), and immune system components (T cells; macrophages) (1, 5). About 50% of plasma plasminogen circulates bound to HPRG. Upon immobilization to cell surface tropomyosin in a Zn++‑dependent manner, it is converted to plasmin by tPA (7 - 9). HPRG also shows antiangiogenic activity on endothelial cells (10). Finally, it binds to cytoplasmic ligand(s) exposed during cellular necrosis, and facilitates macrophage phagocytosis (11).
Jones, A.L. et al. (2005) Immunol. Cell Biol. 83:106.
Koide, T. and S. Odani (1987) FEBS Lett. 216:17.
Hulett, M.D. and C.R. Parish (2000) Immunol. Cell Biol. 78:280.
GenBank Accession # NP_444406.
Borza, D-B. et al. (1996) Biochemistry 35:1925.
Sorensen, C.B. et al. (1993) FEBS Lett. 328:285.
Donate, F. et al. (2004) Cancer Res. 64:5812.
Borza, D-B. and W.T. Morgan (1997) J. Biol. Chem. 272:5718.
Guan, X. et al. (2004) Thromb. Haemost. 92:403.
Juarez, J.C. et al. (2002) Cancer Res. 62:5344.
Jones, A.L. et al. (2005) J. Biol. Chem. 280:35733.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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