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Recombinant Mouse HGF Activator Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1200-SE

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1200-SE-010

Key Product Details

Source

NS0

Accession #

Structure / Form

Active form

Conjugate

Unconjugated

Applications

Enzyme Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse HGF Activator protein
Met1-Ser653 with a C-terminal 10-His tag
The pro form was purified, activated and further purified.

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Val370 & Ile406

Predicted Molecular Mass

32 kDa & 28 kDa

SDS-PAGE

35 kDa and 32 kDa, reducing conditions

Activity

Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The specific activity is >100 pmol/min/µg, as measured under the described conditions.

Formulation, Preparation and Storage

1200-SE
Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and CaCl2.
Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.

Background: HGF Activator

Hepatocyte Growth Factor Activator (HGFA) is a serine endopeptidase that cleaves at the peptide bond between Arg494 and Val495 of single-chain human HGF precursor, generating the active heterodimer (1). HGFA is produced and secreted by the liver and normally circulates in the blood as an inactive zymogen (2, 3). The zymogen has a weak affinity for heparin but acquires a strong affinity for heparin upon activation that is linked to blood coagulation. This property may ensure the local action of this enzyme at the site of tissue injury (3). Mouse HGFA precursor (653 amino acid residues) contains several predicted domains including a signal peptide (residues 1‑29), a propeptide (residues 30‑369), and a mature and active form (residues 370 to 653) that is further processed into a short chain (residues 370‑405) and a long chain (residues 406‑653). The short chain and the long chain (catalytic domain) may form a disulfide bond linked dimer. HGFA can be activated by autocatalysis or by thrombin (4). The active protease can be inhibited by HGFA inhibitors (HAIs). Two HAIs, HAI-1 and HAI-2, are known in mouse and human. HAI-1 is not only an inhibitor, but also a specific acceptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (5).

References

  1. Kitamura, N. (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. Eds.), p. 1712, Academic Press, San Diego.
  2. Miyazawa, K. et al. (1993) J. Biol. Chem. 268:10024.
  3. Miyazawa, K. et al. (1996) J. Biol. Chem. 271:3615.
  4. Shimomura, T. et al. (1993) J. Biol. Chem. 268:22927.
  5. Kataoka, H. et al. (2000) J. Biol. Chem. 275:40453.

Long Name

Hepatocyte Growth Factor Activator

Alternate Names

HGFA, Hgfac

Entrez Gene IDs

3083 (Human); 54426 (Mouse)

Gene Symbol

HGFAC

UniProt

Additional HGF Activator Products

Product Documents for Recombinant Mouse HGF Activator Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse HGF Activator Protein, CF

For research use only

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