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Recombinant Mouse HAI-2B Protein, CF

Bio-Techne includes R&D Systems | Catalog # 1107-PI

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1107-PI-010

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Inhibition Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse HAI-2B protein
Arg30-Lys140, with a C-terminal 10-His tag

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Arg30

Predicted Molecular Mass

14 kDa

SDS-PAGE

23 kDa, reducing conditions

Activity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002).
The IC50 value is <2 nM, as measured under the described conditions.

Formulation, Preparation and Storage

1107-PI
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 100 μg/mL in sterile, deionized water.

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Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 6 months from date of receipt, -20 to -70 °C as supplied.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-2B

Three alternatively spliced forms of HAI-2 have been found in mouse tissues (1). HAI-2A, the full-length molecule, consists of two Kunitz domains and a C-terminal transmembrane domain. HAI-2A is a major form expressed in human tissues, but a minor form in mouse tissues. HAI-2B, with residues 37‑93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin (2).

References

  1. Itoh, H. et al. (1999) Biochem. Biophys. Res. Comm. 255:740.
  2. Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.

Long Name

HGF Activator Inhibitor Type 2B

Alternate Names

HAI2B

Entrez Gene IDs

20733 (Mouse)

Gene Symbol

SPINT2

UniProt

Additional HAI-2B Products

Product Documents for Recombinant Mouse HAI-2B Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse HAI-2B Protein, CF

For research use only

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