Mouse myeloma cell line, NS0-derived mouse HAI-2B protein Arg30-Lys140, with a C-terminal 10-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<1.0 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
23 kDa, reducing conditions
Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (Catalog # ES002). The IC50 value is <2 nM, as measured under the described conditions.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile, deionized water.
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
6 months from date of receipt, -20 to -70 °C as supplied.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Three alternatively spliced forms of HAI-2 have been found in mouse tissues (1). HAI-2A, the full-length molecule, consists of two Kunitz domains and a C-terminal transmembrane domain. HAI-2A is a major form expressed in human tissues, but a minor form in mouse tissues. HAI-2B, with residues 37‑93 spliced out and thus lack of the first Kunitz domain, is a major form in mouse tissues. HAI-2B is an effective inhibitor of both HGF activator and trypsin (2).
Itoh, H. et al. (1999) Biochem. Biophys. Res. Comm. 255:740.
Kataoka, H. et al. (2002) Biochem. Biophys. Res. Comm. 290:1096.
HGF Activator Inhibitor Type 2B
Entrez Gene IDs
Citations for Recombinant Mouse HAI-2B Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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