Source	Mouse myeloma cell line, NS0-derived mouse GFR alpha-1/GDNF R alpha-1 protein Asp25-Ser430, with a C-terminal 6-His tag
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	Ala19
Predicted Molecular Mass	47 kDa
SDS-PAGE	57-67 kDa, reducing conditions
Activity	Measured by its binding ability in a functional ELISA. When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 μL/well), the concentration of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 that produces 50% optimal binding response is 0.04-0.24 μg/mL.

Recombinant Mouse GFR alpha-1 His-tag Protein, CF Scientific Data Examples

	Recombinant Mouse GFR alpha-1 His-tag Protein Binding Activity When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 µL/well), Recombinant Mouse GFRa-1/GDNF Ra-1 (Catalog # 10070-GR) binds with an ED₅₀ of 0.04-0.24 µg/mL.
	Recombinant Mouse GFR alpha-1 His-tag Protein SDS-PAGE 2 μg/lane of Recombinant Mouse GFR alpha ‑1/GDNF R alpha ‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57 - 67 kDa and 43 - 57 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10070-GR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: GFR alpha-1/GDNF R alpha-1

Mouse GFR alpha -1/GDNF R alpha -1 contains a 24 amino acid (aa) signal peptide, a 406 aa GDNF receptor domain, and a 38 aa propeptide. Like other GPI-linked proteins, mouse GFR alpha -1/GDNF R alpha -1 has a C‑terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Mouse homolog's GDNF receptor domain contains 99% sequence identity with its rat homolog, and 95% sequence identity with its human homolog. The expression of the various GFR alpha s are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alpha s in mediating the activities of the GDNF family of neurotrophic factors (1, 2). GFR alpha -1/GDNF R alpha -1 is expressed by neuronal cells, Schwann cells, and injured sciatic nerve where it recruits c-Ret to the lipid rafts (4). It is also over-expressed in a subset of estrogen responsive breast cancers, where it enhances proliferation and cell scattering in response to GDNF (5). GDNF/GFR alpha -1/c-Ret signaling pathway is part of an inflammatory response in breast cancer. TNF alpha and IL-1 beta enhance expression of GDNF in breast cancer tissue (5). Recent studies have discovered that GFR alpha -1/GDNF R alpha -1 expression is non-existent in Alzheimer brain neurons, which leads to no axonal regrowth and apoptosis (6). Addition of exogenous GFR alpha -1/GDNF R alpha -1 to the Alzheimer brain neurons was able to restore survival and axonal regrowth (6).

Item	Value
References	Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:5801. Paratcha, G. et al. (2001) Neuron 29:171. Esseghir, S. et al. (2007) Cancer Res. 67:11732. Konishi, Y. et al. (2014) J Neurosci. 34:13127.
Long Name	Glial Cell line-derived Neurotrophic Factor Receptor alpha 1
Entrez Gene IDs	2674 (Human); 14585 (Mouse); 25454 (Rat)
Alternate Names	DKFZp313E1012, DKFZp686J0156, GDNF R alpha-1, GDNF family receptor alpha 1, GDNF family receptor alpha-1, GDNF receptor alpha-1, GDNFR-alpha-1, GDNFRAGFR-ALPHA-1, GDNFRMGC23045, GFR alpha-1, GFR alpha1, GFR-alpha-1, GFRA1, GFRa-1, GPI-linked anchor protein, Glial cell line-derived neurotrophic factor receptor alpha, PI-linked cell-surface accessory protein, RET ligand 1, RET1L, RETL1FLJ10561, TGF-beta related neurotrophic factor receptor 1, TGF-beta-related neurotrophic factor receptor 1, TRNR1FLJ31546

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Recombinant Mouse GFR alpha-1 His-tag Protein, CF

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