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Recombinant Mouse GFR alpha-1 His-tag Protein, CF

Catalog # 10070-GR | R&D Systems, Inc. a Bio-Techne Brand
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10070-GR-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Monomer

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse GFR alpha-1/GDNF R alpha-1 protein
Asp25-Ser430, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala19

Predicted Molecular Mass

47 kDa

SDS-PAGE

57-67 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 μL/well), the concentration of Recombinant Mouse GFR alpha‑1/GDNF R alpha‑1 that produces 50% optimal binding response is 0.04-0.24 μg/mL.

Scientific Data Images for Recombinant Mouse GFR alpha-1 His-tag Protein, CF

Recombinant Mouse GFR alpha-1 His-tag Protein Binding Activity

Recombinant Mouse GFR alpha-1 His-tag Protein Binding Activity

When Recombinant Rat GDNF (Catalog # 512-GF) is coated at 25 ng/mL (100 µL/well), Recombinant Mouse GFRa-1/GDNF Ra-1 (Catalog # 10070-GR) binds with an ED50 of 0.04-0.24 µg/mL.
Recombinant Mouse GFR alpha-1 His-tag Protein SDS-PAGE

Recombinant Mouse GFR alpha-1 His-tag Protein SDS-PAGE

2 μg/lane of Recombinant Mouse GFR alpha‑1/GDNF R alpha‑1 was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 57 - 67 kDa and 43 - 57 kDa, respectively.

Formulation, Preparation and Storage

10070-GR
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, ≤ -20 °C under sterile conditions after reconstitution.

Background: GFR alpha-1/GDNF R alpha-1

Mouse GFR alpha-1/GDNF R alpha-1 contains a 24 amino acid (aa) signal peptide, a 406 aa GDNF receptor domain, and a 38 aa propeptide. Like other GPI-linked proteins, mouse GFR alpha-1/GDNF R alpha-1 has a C‑terminal hydrophobic region which is preceded by a three aa residue (ASS) GPI-binding site. Mouse homolog's GDNF receptor domain contains 99% sequence identity with its rat homolog, and 95% sequence identity with its human homolog. The expression of the various GFR alphas are differentially regulated in the central and peripheral nervous system, suggesting complementary roles for the GFR alphas in mediating the activities of the GDNF family of neurotrophic factors (1, 2). GFR alpha-1/GDNF R alpha-1 is expressed by neuronal cells, Schwann cells, and injured sciatic nerve where it recruits c-Ret to the lipid rafts (4). It is also over-expressed in a subset of estrogen responsive breast cancers, where it enhances proliferation and cell scattering in response to GDNF (5). GDNF/GFR alpha-1/c-Ret signaling pathway is part of an inflammatory response in breast cancer. TNF alpha and IL-1 beta enhance expression of GDNF in breast cancer tissue (5). Recent studies have discovered that GFR alpha-1/GDNF R alpha-1 expression is non-existent in Alzheimer brain neurons, which leads to no axonal regrowth and apoptosis (6). Addition of exogenous GFR alpha-1/GDNF R alpha-1 to the Alzheimer brain neurons was able to restore survival and axonal regrowth (6).

References

  1. Thompson, J. et al. (1998) Mol. Cell Neurosci. 11:117.
  2. Trupp, M. et al. (1998) Mol. Cell Neurosci. 11:47.
  3. Baloh, R.H. et al. (1998) Proc. Natl. Acad. Sci. U.S.A. 95:5801.
  4. Paratcha, G. et al. (2001) Neuron 29:171.
  5. Esseghir, S. et al. (2007) Cancer Res. 67:11732.
  6. Konishi, Y. et al. (2014) J Neurosci. 34:13127.

Long Name

Glial Cell line-derived Neurotrophic Factor Receptor alpha 1

Alternate Names

GDNF R alpha-1, GFR alpha1, GFRa-1, GFRA1

Entrez Gene IDs

2674 (Human); 14585 (Mouse); 25454 (Rat)

Gene Symbol

GFRA1

UniProt

Product Documents for Recombinant Mouse GFR alpha-1 His-tag Protein, CF

Certificate of Analysis

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Product Specific Notices for Recombinant Mouse GFR alpha-1 His-tag Protein, CF

For research use only

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