Product Specifications for Recombinant Mouse Gas1 Protein, CF
Mouse myeloma cell line, NS0-derived mouse Gas1 protein Leu39-Ser315 & Arg43-Ser315, both with a C-terminal 6-His tag
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
<0.01 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Leu39 & Arg43
Predicted Molecular Mass
40 kDa, reducing conditions
Measured by its binding ability in a functional ELISA. Immobilized rmGas1 at 1 µg/mL (100 µL/well) can bind rmArtemin at a linear range of 0.1-10 ng/mL.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 100 μg/mL in sterile PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
Gas1 (Growth Arrest Specific 1) is one of six structurally unrelated proteins that were identified by their increased expression in growth-arrested cells relative to actively proliferating cells (1, 2). Following mitogenic stimulation, Gas1 expression is transcriptionally suppressed by c-Myc as cells transit from G0 to G1 phases of the cell cycle (3, 4). Overexpression of Gas1 prevents S phase entry and DNA synthesis (5). Gas1-mediated blockade of the cell cycle is p53-dependent but does not require the transactivating domain of p53 (6). The mouse Gas1 cDNA encodes a 343 amino acid (aa) precursor that includes a 38 aa signal sequence, a 277 aa mature protein, and a 28 aa C-terminal propeptide. Gas1 contains Ala-rich and Asp-rich regions as well as an RGD sequence (5). Mature mouse and human Gas1 share 85% aa sequence identity. Mouse Gas1 is a 40 kDa GPI linked glycoprotein that is uniformly distributed on the cell surface (7). In contact inhibited vascular endothelial cells, Gas1 is induced by VE-Cadherin and VEGF expression and mediates the anti-apoptotic effect of VEGF (8). In contrast, Gas1 is induced in hippocampal neurons after NMDA exposure but functions as a pro-apoptotic effector of NMDA-mediated excitotoxicity (9). Gas1 exhibits a range of developmental effects including either promoting or inhibiting growth and differentiation of somite, limb, cerebellar, and eye tissues (10 - 14). Gas1 mediates the antagonistic effect of Wnt proteins toward Shh function by binding the N-terminal region of Shh (11). The dependence of Gas1 functions on the cellular context has been addressed by suggesting that Gas1 could function as a co-receptor for GDNF family ligands (15). This speculation is supported by R&D Systems’ data that demonstrate direct binding of Gas1 to Artemin and Neurturin.
Schneider, C. et al. (1988) Cell 54:787.
Mullor, J.L. and A.R. Altaba (2002) BioEssays 24:22.
Del Sal, G. et al. (1994) Proc. Natl. Acad. Sci. USA 91:1848.
Lee, T.C. et al. (1997) Proc. Natl. Acad. Sci. USA 94:12886.
Del Sal, G. et al. (1992) Cell 70:595.
Del Sal, G. et al. (1995) Mol. Cell. Biol. 15:7152.
Stebel, M. et al. (2000) FEBS Lett. 481:152.
Spagnuolo, R. et al. (2004) Blood 103:3005.
Mellstrom, B. et al. (2002) Mol. Cell Neurosci. 19:417.
Lee, K.K.H. et al. (2001) Dev. Biol. 234:188.
Lee, C.S. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11347.
Liu, Y. et al. (2002) Development 129:5289.
Liu, Y. et al. (2001) Dev. Biol. 236:30.
Lee, C.S. et al. (2001) Dev. Biol. 236:17.
Schueler-Furman, O. et al. (2006) Trends Pharmacol. Sci. 27:72.
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
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