Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF

Name: Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF
Brand: R&D Systems
SKU: 9039-GAB

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 9039-GAB

Citations (1)

Product Datasheet / COA / SDS

Catalog #

Availability

Size / Price

Qty

Setting the standard in quality research reagents

Key Product Details

Source:	HEK293
Accession #:	NP_034835
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF

Complete Your Research

Product Specifications for Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF

Source	Human embryonic kidney cell, HEK293-derived mouse Galectin-3 protein Met1-Ile264
Purity	>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin level	<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis	No results obtained
Predicted Molecular Mass	27 kDa
SDS-PAGE	27-36 kDa, reducing conditions
Activity	Measured by the ability of the immobilized protein to support the adhesion of D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 1-5 μg/mL.

Scientific Data Examples for Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF

	Recombinant Mouse Galectin-3 (Hek293-expressed) Protein Bioactivity Recombinant Mouse Galectin-3 (Catalog # 9039-GAB) supports the adhesion of D10.G4.1 mouse helper T cells. The ED₅₀ for this effect is 1-5 μg/mL.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

9039-GAB

Formulation	Lyophilized from a 0.2 μm filtered solution in HEPES, NaCl, TCEP, PEG and Trehalose.
Reconstitution	Reconstitute at 200 μg/mL in water.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Galectin-3

Galectin-3, also known as Mac-2, L29, CBP35, and epsilon BP, is classified as a chimeric member of the Galectin superfamily and contains one carbohydrate recognition domain (CRD) linked to a non-lectin domain (1, 2). Mature mouse Galectin-3 shares 80% and 86% amino acid (aa) sequence identity with human and rat Galectin-3, respectively. Galectin-3 is a 26-kDa protein that can be nuclear, cytoplasmic, or secreted (3, 4). Nuclear Galectin-3 can modulate gene expression, while cytosolic Galectin-3 can inhibit apoptosis and can participate in exocytosis, Caveolin-mediated endocytosis, and macrophage-mediated clearance of apoptotic cells (5-7). Extracellular Galectin-3 has been shown to form high-order oligomers that promote the crosslinking of cell surface oligosaccharides as well as integrin-dependent cell adhesion and apoptosis (8-11). Galectin-3 contributes to the innate immune response against Candida albicans and Streptococcus pneumoniae, and it can facilitate acute inflammatory responses via neutrophil activation and opsonization, macrophage recruitment, and mast cell activation (12-14). Galectin-3 can also contribute to chronic inflammation and fibrosis (15). It is implicated in neuroinflammatory disorders of the central nervous system, cardiac fibrosis, and heart failure, as well as tumor growth, progression, and metastasis (16-18).

Item	Value
References	Robertson, M.W. et al. (1990) Biochemistry 29:8093. Elola, M.T. et al. (2007) Cell. Mol. Life Sci. 64:1679. Haudek, K.C. et al. (2010) Biochim. Biophys. Acta 1800:181. Hughes, R.C. (1999) Biochim. Biophys. Acta 1473:172. Krzeslak, A. and A. Lipinska (2004) Cell. Mol. Biol. Lett. 9:305. Dumic, J. et al. (2006) Biochim. Biophys. Acta 1760:616. Sano, H. et al. (2003) J. Clin. Invest. 112:389. Ahmad, N. et al. (2004) J. Biol. Chem. 279:10841. Friedrichs, J. et al. (2008) J. Biol. Chem. 283:32264. Wen, Y. et al. (2006) J. Cell. Biochem. 98:115. Zhuo, Y. et al. (2008) J. Biol. Chem. 283:22177. Henderson, N.C. and T. Sethi (2009) Immunol. Rev. 230:160. Kohatsu, L. et al. (2006) J. Immunol. 177:471814. Nieminen, J. et al. (2008) J. Immunol. 180:2466. Henderson, N.C. et al. (2008) Am. J. Pathol. 172:288. Newlaczyl, A.U. and L.G. Yu (2011) Cancer Lett. 313:123. Sherwi, N. et al. (2012) Future Cardiol. 8:885. Shin, T. (2013) Acta Histochem. 115:407.
Entrez Gene IDs	3958 (Human); 16854 (Mouse)
Alternate Names	AGE-R3, CBP 35,35 kDa lectin, CBP35, Carbohydrate-binding protein 35, GAL3, GALBPCBP35, GALIG, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, Galectin-3, Galectin3, IgE-binding protein, L-31, L29, L31, LGALS2, LGALS3, Laminin-binding protein, Lectin L-29, MAC2GAL3, Mac-2, Mac-2 antigen, galactin-3, lectin, galactoside-binding, soluble, 3

Citations for Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF (1)

Showing 1 - 1 of 1 citation Showing All

Filter By:

Search Title:

Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Galectin-3 (Hek293-expressed) Protein, CF include:

Species: Mouse

Applications: Cell Culture

T Li et al. (2022), Thiamine pretreatment improves endotoxemia-related liver injury and cholestatic complications by regulating galactose metabolism and inhibiting macrophage activation International immunopharmacology, 108(0):108892.
PMID: 35640376

There are no citations that match your criteria.