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Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 112-FZ

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112-FZ-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Bioactivity, Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-8 protein
Mouse Frizzled-8
(Ala25-Pro173) &

(Ala28-Pro173)
Accession # Q61091

IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala25 & Ala28

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

59-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells.
The ED50 for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).

Measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA with an estimated Kd <1 nM.

Formulation, Preparation and Storage

112-FZ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 250 μg/mL in sterile PBS.

Reconstitution Buffer Available:
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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-8

Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1, 2). Frizzled proteins are thought to be G‑protein‑coupled (1). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP‑6 acting as co‑receptors, stabilizes beta-catenin and promotes gene transcription that is important in development and tissue maintenance (1). Mouse Frizzled-8 cDNA encodes 685 amino acids (aa) including a 27 aa signal peptide, a 244 aa extracellular domain (ECD), the 7TM region (aa 272-603), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa 604-685) (2). The ECD includes a cysteine-rich region (CRD, aa 30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa 25-173, mouse Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with human, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 3). During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis (4). Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate beta-catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein-4) or CTGF (connective tissue growth factor) inhibits Wnt signaling (5‑7). These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas (5‑10).

References

  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
  3. Dann, C.E. et al. (2001) Nature 412:86.
  4. Lu C.C. et al. (2004) Gene Expr. Patterns 4:569.
  5. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247.
  6. Zhu, W. et al. (2008) Nature 454:345.
  7. Mercurio, S. et al. (2004) Development 131:2137.
  8. Chesnutt, C. et al. (2004) Dev. Biol. 274:334.
  9. Yamashita, J.K. et al. (2005) FASEB J. 19:1534.
  10. DeAlmeida, V.I. et al. (2007) Cancer Res. 67:5371.

Alternate Names

Frizzled8, FZD8

Entrez Gene IDs

8325 (Human); 14370 (Mouse)

Gene Symbol

FZD8

UniProt

Additional Frizzled-8 Products

Product Documents for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

For research use only

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