Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

Name: Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 112-FZ
Price: 460 USD

R&D Systems, Inc. a Bio-Techne Brand

Catalog # 112-FZ

Citations (3)

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Key Product Details

Source:	NS0
Accession #:	Q61091
Structure / Form:	Disulfide-linked homodimer
Applications:	Binding Activity, Bioactivity

View Full Technical Details for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

Product Specifications for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-8 protein

Mouse Frizzled-8 (Ala25-Pro173) & (Ala28-Pro173) Accession # Q61091	IEGRMD	Human IgG1 (Pro100-Lys330)
N-terminus		C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain

Endotoxin level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Ala25 & Ala28

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

59-60 kDa, reducing conditions

Activity

Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED₅₀ for this effect is 15-75 ng/mL in the presence of 10 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN). Measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA with an estimated K_d <1 nM.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

112-FZ

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 250 μg/mL in sterile PBS. Reconstitution Buffer Available: Size / Price Qty Loading... RB01:
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Frizzled-8

Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors (1, 2). Frizzled proteins are thought to be G‑protein‑coupled (1). Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP‑6 acting as co‑receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance (1). Mouse Frizzled-8 cDNA encodes 685 amino acids (aa) including a 27 aa signal peptide, a 244 aa extracellular domain (ECD), the 7TM region (aa 272-603), and a C-terminal cytoplasmic domain with a PDZ binding motif (aa 604-685) (2). The ECD includes a cysteine-rich region (CRD, aa 30-151) that binds Wnts and is highly conserved among Frizzleds, and a linker region. Within aa 25-173, mouse Frizzled-8 shares 99%, 99%, 90% and 85% aa identity with human, rat, Xenopus and zebrafish Frizzled-8, respectively. It also shares 82% aa identity with human Frizzled-5. Frizzleds can form homodimers or selective hetero-oligomers with other family members, which can involve the TM regions and possibly the CRD (1, 3). During mouse development, Frizzled-8 is expressed in tissues that are important for organizing the anterior-posterior axis (4). Interactions of Frizzled-8 with several non-Wnt ligands have been identified. Interaction with Frizzled-8 and LRP-6 allows R-spondins to activate beta -catenin signaling pathways, while interaction with IGFBP-4 (insulin-like growth factor binding protein-4) or CTGF (connective tissue growth factor) inhibits Wnt signaling (5‑7). These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas (5‑10).

Item	Value
References	Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468. Dann, C.E. et al. (2001) Nature 412:86. Lu C.C. et al. (2004) Gene Expr. Patterns 4:569. Nam, J-S. et al. (2006) J. Biol. Chem. 281:13247. Zhu, W. et al. (2008) Nature 454:345. Mercurio, S. et al. (2004) Development 131:2137. Chesnutt, C. et al. (2004) Dev. Biol. 274:334. Yamashita, J.K. et al. (2005) FASEB J. 19:1534. DeAlmeida, V.I. et al. (2007) Cancer Res. 67:5371.
Entrez Gene IDs	8325 (Human); 14370 (Mouse)
Alternate Names	FZ-8, FZD8, Frizzled-8, Frizzled8, frizzled (Drosophila) homolog 8, frizzled 8, frizzled family receptor 8, frizzled homolog 8 (Drosophila), hFZ8

Citations for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF (3)

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Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF include:

Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Esen E et al. (2015-01-01), PTH Promotes Bone Anabolism by Stimulating Aerobic Glycolysis via IGF Signaling. J Bone Miner Res, 30(11):1959-68.
PMID: 25990470
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Lindsley RC et al. (2006-01-01), Canonical Wnt signaling is required for development of embryonic stem cell-derived mesoderm. Development, 133(19):3787-96.
PMID: 16943279
Species: Mouse
Sample Types: Whole Tissue
Applications: Bioassay

Viti J et al. (2003-01-01), Wnt regulation of progenitor maturation in the cortex depends on Shh or fibroblast growth factor 2. J. Neurosci., 23(13):5919-27.
PMID: 12843296

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Recombinant Mouse Frizzled-8 Fc Chimera Protein, CF

R&D Systems, Inc. a Bio-Techne Brand

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