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Recombinant Mouse Frizzled-7 Fc Chimera Protein, CF

Bio-Techne includes R&D Systems | Catalog # 198-FZ

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198-FZ-050

Key Product Details

Source

NS0

Accession #

Structure / Form

Disulfide-linked homodimer

Conjugate

Unconjugated

Applications

Binding Activity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse Frizzled-7 protein
Mouse Frizzled-7
(Gln33 - Leu185)
Accession # Q61090
IEGRMD Human IgG1
(Pro100 - Lys330)
N-terminus C-terminus

Purity

>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

No results obtained: Gln33 predicted

Predicted Molecular Mass

43 kDa (monomer)

SDS-PAGE

55-60 kDa, reducing conditions

Activity

Measured by its ability to bind biotinylated Wnt-3a in a functional ELISA with an estimated KD 

Formulation, Preparation and Storage

198-FZ
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution
Reconstitute at 200 μg/mL in sterile PBS.

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Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Frizzled-7

Frizzled-7 is a member of the Frizzled family of unconventional G-protein-coupled glycoprotein receptors for the Wnt signaling pathway (1 ‑ 3). The Wnt genes encode a large family of glycoproteins that are essential in development and tissue maintenance (1, 2). Like other Frizzled family members, mouse Frizzled-7 contains a divergent N‑terminal signal peptide (amino acid (aa) 1 ‑ 32), a highly conserved extracellular cysteine-rich domain (CRD, aa 44 ‑ 169), a variable-length linker region (aa 170 ‑ 254), a seven-pass transmembrane region (aa 255 ‑ 547), and a variable-length C-terminal cytoplasmic domain (aa 548 ‑ 572) (1 ‑ 4). The CRD, which comprises the binding site for Wnts and other ligands such as Syndecan 4 and fibronectin, spans about 130 amino acid residues and contains ten invariant cysteine residues (2 ‑ 4). Expressed alone, it can compete with native Frizzled to inhibit Wnt canonical signaling (5). Within aa 33 ‑ 185, mouse Frizzled-7 shares ~99% aa identity with human, rat, canine and bovine Frizzled-7. Mature Frizzled-7 also shares 80% aa identity with Frizzled-1 and Frizzled-2. Roles for Frizzled-7 have been determined in both canonical Wnt/ beta-Catenin-mediated signaling and non‑canonical planar cell polarity and calcium pathways (1, 2, 5). During development, Frizzled-7 is expressed during gastrulation and in the fetal gut, kidney and lung where it is thought to influence tissue morphogenesis via non‑canonical signaling pathways (3 ‑ 6). In the adult, Frizzled-7 is expressed in skeletal muscle, especially in satellite cells that mediate muscle regeneration in response to Wnt7a (3, 4, 7). It is expressed in embryonic stem cells (ES), contributing to self-renewal signaling (8). It has been implicated in mesenchymal-to-epithelial transition in colorectal cancer (2, 9). Frizzled-7 mRNA has also been detected in adult heart and placenta (4).

References

  1. Schulte, G. and V. Bryja (2007) Trends Pharmacol. Sci. 28:518.
  2. Vincan, E. et al. (2007) Front. Biosci. 12:4558.
  3. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
  4. Sagara, N. et al. (1998) Biochem. Biophys. Res. Commun. 252:117.
  5. Medina, A. et al. (2000) Mech. Dev. 92:227.
  6. Kemp, C.R. et al. (2007) Dev. Dyn. 236:2011.
  7. Le Grand, F. et al. (2009) Cell Stem Cell 4:535.
  8. Melchior, K. et al. (2008) Biol. Chem. 389:897.
  9. Vincan, E. et al. (2007) Oncogene 26:2340.

Alternate Names

Frizzled7, FZD7

Entrez Gene IDs

8324 (Human); 14369 (Mouse)

Gene Symbol

FZD7

UniProt

Additional Frizzled-7 Products

Product Documents for Recombinant Mouse Frizzled-7 Fc Chimera Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse Frizzled-7 Fc Chimera Protein, CF

For research use only

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