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Recombinant Mouse FLRT3 His-tag Protein, CF

Catalog # 10614-FL | R&D Systems, Inc. a Bio-Techne Brand
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10614-FL-050

Key Product Details

Source

NS0

Accession #

Conjugate

Unconjugated

Applications

Bioactivity

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FLRT3 protein
Lys29-Pro528, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Lys29

Predicted Molecular Mass

57 kDa

SDS-PAGE

67-87 kDa, under reducing conditions

Activity

Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells.
The ED50 for this effect is 0.4-3.6 μg/mL.

Scientific Data Images for Recombinant Mouse FLRT3 His-tag Protein, CF

Recombinant Mouse FLRT3 His-tag Protein Bioactivity.

Recombinant Mouse FLRT3 His-tag (Catalog # 10614-FL) supports the adhesion of Neuro 2A mouse neuroblastoma cells. The ED50 for this effect is 0.4-3.6 µg/mL.

Recombinant Mouse FLRT3 His-tag Protein SDS-PAGE.

2 μg/lane of Recombinant Mouse FLRT3 His-tag Protein (Catalog # 10614-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 67-87 kDa.

Formulation, Preparation and Storage

10614-FL
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 500 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: FLRT3

Fibronectin-like domain-containing leucine-rich transmembrane protein 3 (FLRT3) is one of three FLRT glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). Mouse FLRT3 consists of an extracellular domain (ECD) containing 10 leucine-rich repeats (LRR) and a juxtamembrane fibronectin type III domain, a single transmembrane domain and a cytoplasmic domain (1). The ECD of mature mouse FLRT3 shares 96% amino acid (aa) sequence identity with the ECD of human and rat FLRT3. In mouse embryos, FLRT3 is required to maintain tissue integrity and organize the underlying basement membrane in the anterior visceral endoderm (AVE) (2). The fibronectin domain is responsible for binding to FGF receptors and is thought to regulate FGF signaling during development (3, 4). The LRR domains are responsible for both the localization in areas of cell contact and homotypic cell-cell association (5). This may be through direct interaction with other FLRT molecules, or alternatively, by regulating internalization of adhesion molecules such as cadherins (5, 6). Developmentally, FLRT3 is located in somitic regions on dermatomyotomal muscle precursors and myotomal cells before their migration to the myotome and syndetome, respectively (3). FLRT3 is also expressed at the midbrain/hindbrain boundary and in the apical ectodermal ridge where it may influence FGF signaling (3). Further, FLRT3 is up-regulated and promotes neurite outgrowth following experimental peripheral nerve injury in rats (7, 8). Genetic deletion in mouse embryos results in defective headfold fusion and endoderm migration (9). FLRT3 is a ligand to LPHN1 and maybe involved in the Tim-3-galectin-9 pathway (10). Elevated expression of FLRT3 has been found in breast cancer cells and may serve as a biomarker for head and neck squamous cell carcinoma (HNSCC) (10, 11).

References

  1. Lacy, S.E. et al. (1999) Genomics 62:417.
  2. Egea, et al. (2008) Genes Dev.22:3349.
  3. Haines, B.P. et al. (2006) Dev. Biol. 297:14.
  4. Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
  5. Karaulanov, E.E. et al. (2006) EMBO Rep. 7:283.
  6. Ogata, S. et al. (2007) Genes Dev. 21:1817.
  7. Tsuji, L. et al. (2004) Biochem. Biophys. Res. Commun. 313:1086.
  8. Robinson, M. et al. (2004) Mol. Cell. Neurosci. 27:202.
  9. Maretto, S. et al. (2008) Dev. Biol. 318:184.
  10. Yasinska, I.M. et al. (2019) Front. Immunol. 10:1594.
  11. Tripathi, N. et al. (2020) J Cell Physiol. 235:9922.

Long Name

Fibronectin Leucine Rich Transmembrane Protein 3

Alternate Names

fibronectin leucine rich transmembrane protein 3, Fibronectin-like domain-containing leucine-rich transmembrane protein 3, HH21, KIAA1469, leucine-rich repeat transmembrane protein FLRT3

Entrez Gene IDs

23767 (Human); 71436 (Mouse)

Gene Symbol

FLRT3

UniProt

Additional FLRT3 Products

Product Documents for Recombinant Mouse FLRT3 His-tag Protein, CF

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Recombinant Mouse FLRT3 His-tag Protein, CF

For research use only

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