Mouse myeloma cell line, NS0-derived mouse FLRT2 protein Cys36-Ser539, with a C-terminal 6-His tag
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
<0.10 EU per 1 μg of the protein by the LAL method.
N-terminal sequence Analysis
Predicted Molecular Mass
71-80 kDa, under reducing conditions
Measured by the ability of the immobilized protein to support the adhesion of Neuro‑2A mouse neuroblastoma cells. The ED50 for this effect is 0.60-4.80 µg/mL.
Recombinant Mouse FLRT2 His-tag Protein, CF Scientific Data Examples
Recombinant Mouse FLRT2 His-tag Protein Bioactivity.
Recombinant Mouse FLRT2 His-tag (Catalog # 10715-FL) supports the adhesion of Neuro 2A mouse neuroblastoma cells. The ED50 for this effect is 0.60-4.80 μg/mL.
Recombinant Mouse FLRT2 His-tag Protein SDS-PAGE.
2 μg/lane of Recombinant Mouse FLRT2 His-tag (Catalog # 10715-FL) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® Blue staining, showing bands at 71-80 kDa.
Formulation, Preparation and Storage
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our
Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant
protein to be stored at a more dilute concentration.
The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or
as an ELISA standard.
In contrast, the carrier free protein is recommended for applications, in which the presence of BSA
Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitute at 500 μg/mL in PBS.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
3 months, -20 to -70 °C under sterile conditions after reconstitution.
FLRT2 is one of three FLRT (fibronectin, leucine rich repeat, transmembrane) glycoproteins expressed in distinct areas of the developing brain and other tissues (1, 2). The 85 kDa type I transmembrane (TM) mouse FLRT2 is synthesized as a 660 amino acid (aa) precursor with a 35 aa signal sequence, a 505 aa extracellular domain (ECD), a 21 aa TM segment and a 99 aa cytoplasmic region. The ECD contains 10 N-terminal leucine-rich repeats flanked by cysteine-rich areas, and a juxtamembrane fibronectin type III domain (1). The mouse FLRT2 ECD shares 97% and 99% aa sequence identity with human and rat FLRT2 ECD, respectively. The fibronectin domain of all three FLRTs can bind to FGF receptors (2). This binding is thought to regulate FGF signaling during development (2, 3). The LRR domains are responsible for both the localization of FLRTs in areas of cell contact and homotypic cell-cell association (4). This may be through direct interactions with other FLRT molecules or, as has been shown for FLRT3, by regulating internalization of adhesion molecules such as cadherins (4, 5). In adulthood, FLRT2 mRNA is most abundant in pancreas, but is also present in skeletal muscle, brain and heart (1). FLRT2 in mouse embryos shows highest expression in a subset of the sclerotome in the brain, the stomach, and posterior to the developing heart (2). This expression is distinct from that of FLRT1 and FLRT3 (2). Overexpression of FLRT2 promotes tumor suppression in colorectal and breast cancers (6, 7).
Lacy, S. E. et al. (1999) Genomics 62:417.
Haines, B. P. et al. (2006) Dev. Biol. 297:14.
Bottcher, R.T. et al. (2004) Nat. Cell Biol. 6:38.
Karaulanov, E. E. et al. (2006) EMBO Rep. 7:283.
Ogata, S. et al. (2007) Genes Dev. 21:1817.
Guo, X. et al. (2020) J. Cancer 11:7329.
Bae, H. et al. (2017) Sci. Rep. 7:272.
Fibronectin Leucine Rich Transmembrane Protein 2
Entrez Gene IDs
23768 (Human); 399558 (Mouse)
FLRT2, Fibronectin Leucine Rich Transmembrane Protein 2, Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 2, KIAA0405, Leucine-Rich Repeat Transmembrane Protein FLRT2
Citations for Recombinant Mouse FLRT2 His-tag Protein, CF
Which Brands are Currently Available on bio‑techne.com? R&D Systems, Tocris Bioscience and ProteinSimple branded products are available to purchase through bio‑techne.com. ProteinSimple branded instruments are available to quote. ACD branded products will be available on bio‑techne.com in the near future. Novus Biologicals branded products are not currently available on bio‑techne.com and can be found at novusbio.com.
Setting the standard in quality research reagents for over 30 years
A trusted leader in quality life science reagents
Your trusted supplier for innovative and high performance life science reagents
Proprietary systems and consumables for simpler, more quantitative and affordable protein analysis
Proprietary RNAscope® technology capable of detecting and quantifying RNA biomarkers in situ at single molecule sensitivity
A world leader in developing liquid biopsy based diagnostics