Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Name: Recombinant Mouse FGFR1 Fc Chimera Protein, CF
Brand: R&D Systems
SKU: 10135-FR

Catalog # 10135-FR

Product Datasheet / COA / SDS

Order Information

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Key Product Details

Source:	NS0
Accession #:	P16092-6
Structure / Form:	Disulfide-linked homodimer
Applications:	Bioactivity

View Full Technical Details for Recombinant Mouse FGFR1 Fc Chimera Protein, CF

Product Specifications

Source

Mouse myeloma cell line, NS0-derived mouse FGFR1 protein

Mouse FGF R1 (Arg22-Ile376) Accession # P16092-6	IEGRMDP	Mouse IgG_2A (Glu98-Lys330)
N-terminus		C-terminus

Purity

>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal sequence Analysis

Arg22

Predicted Molecular Mass

66 kDa

SDS-PAGE

95-115 kDa, under reducing conditions

Activity

Measured by its ability to inhibit FGF acidic-dependent proliferation of NR6R‑3T3 mouse fibroblast cells. The ED₅₀ for this effect is 15-90 ng/mL

Recombinant Mouse FGFR1 Fc Chimera Protein, CF Scientific Data Examples

	Recombinant Mouse FGFR1 Fc Chimera Protein Inhibition Activity Recombinant Mouse FGF R1 Fc Chimera (Catalog # 10135-FR) inhibits Recombinant Mouse FGF acidic (Catalog # 4686-FA) induced proliferation of NR6R-3T3 mouse fibroblast cells. The ED₅₀ for this effect is 15-90 ng/mL.
	Recombinant Mouse FGFR1 Fc Chimera Protein SDS-PAGE 2 μg/lane of Recombinant Mouse FGF R1 Fc Chimer (Catalog # 10135-FR) was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditions and visualized by Coomassie® blue staining, showing bands at 95-115 kDa and 190-230 kDa, respectively.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

10135-FR

Formulation	Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution	Reconstitute at 500 μg/mL in PBS.
Shipping	The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGFR1

Fibroblast growth factor receptor 1 (FGF R1) is a member of a tyrosine kinase family that acts as cell-surface receptors for fibroblast growth factors (FGFs). The FGF R family consists of four members that differ in their ligand affinities and play essential roles in the regulation of embryonic development, cell proliferation, differentiation, and migration (1, 2). Mature mouse FGF R1 consists of a 355 amino acid (aa) extracellular domain (ECD) composed of three immunoglobulin-like domains, a single transmembrane domain and a cytoplasmic tyrosine kinase domain (1). The ECD of mouse FGF R1 shares 98% and 99% aa identity with the human and rat ECD, respectively. FGF R1 binds both acidic and basic fibroblast growth factors and is involved in limb induction (2, 3). Alternative splice variants of this gene have been reported. This product is the longest variant, isoform 1 (1, 4). FGF Rs play essential roles in the regulation of embryonic development, cell proliferation, differentiation and migration (5). Upon FGF and heparan sulfate binding, the FGF Rs form homodimers and the kinase domains are activated (6, 7). Alterations in the binding regulates FGF bioactivity (8).

Item	Value
References	Beer, H.-D. et al. (2000) J. Biol. Chem. 275:16091. Ornitz, D.M. et al. (1992) Mol. Cell. Bio. 12:240. Cuiling, Li. et al. (2005) Dev. 132:4755. Kouhara, H. et al. (1991) Biochem. Biophys. Res. Commun. 176:31. Turner, N and Grose, R. (2010) Nat. Rev. Cancer 10:116. Pantoliano, M.W. et al. (1994) Biochemistry 33:10229. Ornitz, D.M. et al. (1996) J. Biol. Chem. 271:15292. Guerrini, M. et al. (2007) Curr. Pharm. Des. 13:2045.
Long Name	Fibroblast Growth Factor Receptor 1
Entrez Gene IDs	2260 (Human); 14182 (Mouse)
Alternate Names	BFGFR, CD331, CD331 antigen, CEK, EC 2.7.10, EC 2.7.10.1, FGF R1, FGFBR, FGFR-1, FGFR1, FLGH3, FLJ99988, FLT2H4, Flt-2, Fms-like tyrosine kinase 2, H2, H5, HBGFR, KAL 2, KAL2, N-SAM, OGD, Proto-oncogene c-Fgr, bFGF-R-1, basic fibroblast growth factor receptor 1, fibroblast growth factor receptor 1, fms-related tyrosine kinase 2, heparin-binding growth factor receptor, hydroxyaryl-protein kinase, soluble FGFR1 variant 1, soluble FGFR1 variant 2

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